| 光谱学与光谱分析 |
|
|
|
|
|
| An Enzyme Sensor for Phenolic Compounds Analysis |
| LI Ye, YANG Hui, HAN Wei-wei, LIAO Ming-xia, LU Yi-qiang |
| Department of Chemistry, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China |
|
|
|
|
Abstract Phenolic analysis was established on the immobilized horseradish peroxidase (HRP) catalyzed oxidation reaction. It subsequently catalyzed oxidative coupling of phenol with 4-aminophenazone using aqueous hydrogen peroxide to form intensely colored products for spectrophotometric analysis. HRPs were trapped in sol-gel matrix in a mild procedure. The immobilized HRPs maintain almost identical enzymatic activity as those in solution. BET analysis indicates that the silica itself is a porous structure with the average pore diameter of 2.95 nm. It permits small size molecules i.e. hydrogen peroxide, phenol and 4-aminophenazone to diffuse into the matrix while large molecules like enzyme (HRP) remain in the pores. It thus allows a biocatalysis to occur and makes the most out of the enzymes encapsulated in the silica matrix to stand against leakage so that the immobilized horseradish peroxidase could be recycled. The method can be employed for 2-chlorophenol，3-chlorophenol or 2,4-dichlorophenol analysis as well. The feasibility of recycle on immobilized enzyme is evaluated. Although enzymatic activities are obviously decreased after repeated utilization of 9 times, the method definitely offers a potential spectrophotometric biosensor for phenolic compounds analysis.
|
|
Received: 2009-06-29
Accepted: 2009-09-29
|
|
|
|
Corresponding Authors:
LI Ye
E-mail: liye@sas.ustb.edu.cn
|
|
[1] Aitken M D. Journal of Chem. Eng., 1993, 52: B49.
[2] Klibanov A M, Tu T M, Scott K P. Science, 1983, 221: 259.
[3] Proestos C, Sereli D, Komaitis M. Food Chem., 2006, 95: 44.
[4] Schieber A, Keller P, Carle R. J. Chromatogr. A, 2001, 910: 265.
[5] Santos-Buelga C, Williamson G. Methods in Polyphenols Analysis. RSC, Cambridge, UK, 2003.
[6] Hoffmann D, Iinuma Y, Herrmann H. J. Chromatogr. A, 2007, 1143:168.
[7] Chen Z, Xi F, Yang S, et al. Sensor Actuat. B, 2008, 130: 900.
[8] Stevantor R, Fabris S, Momo F. Agric. Food Chem., 2004, 52: 6287.
[9] Diana L D, Lima C A, Duarte V, et al. Talanta, 2007, 72: 1404.
[10] SHI Ming-juan, Aung Myint, DONG Rong-ping, et al(石明娟，Aung Myint, 董永平，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(10): 1945.
[11] Hedges J I, Ertel J R. Anal. Chem., 1982, 54: 174.
[12] Ramos M C, Torijas M C, Navas Díaz A. Sensors Actuat., 2001, 73: 71.
[13] Jin W, Brennan J D. Anal. Chim. Acta, 2002, 461(1): 1.
[14] Carvalho R H, Lemos F, Cabral J M S, et al. J. Mol. Catal. B: Enzym., 2007, 44: 39.
[15] Coradin T, Bah S, Livage J. Colloids Surfaces B, 2004, 35: 53.
[16] Hedges J I, Ertel J R. Anal. Chem., 1982, 54: 174.
[17] Li Y, Yip W T. J. Am. Chem. Soc., 2005, 127: 12756.
[18] Nazari K, Esmaeili N, Mahmoudi A, et al. Enzyme, Microb. Technology, 2007, 41: 226.
[19] Bravoa M N, Silvab S, CoelhobA V, et al. Anal. Chim. Acta, 2006, 563: 23.
|
| [1] |
ZHOU Cai-hua, DING Xiao. DFT Calculation of Absorption Spectra for Planar Porphyrin Derivatives[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1769-1773. |
| [2] |
MA Ping1, 2, Andy Hsitien Shen1*, ZHONG Yuan1, LUO Heng1. Study on UV-Vis Absorption Spectra of Jadeite From Different Origins[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1827-1831. |
| [3] |
JIANG Ya-jing, SONG Jun-ling*, RAO Wei, WANG Kai, LOU Deng-cheng, GUO Jian-yu. Rapid Measurement of Integrated Absorbance of Flow Field Using Extreme Learning Machine[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1346-1352. |
| [4] |
LÜ Yang, PEI Jing-cheng*, GAO Ya-ting, CHEN Bo-yu. Chemical Constituents and Spectra Characterization of Gem-Grade
Triplite[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1204-1208. |
| [5] |
DU Bao-lu, LI Meng, GUO Jin-jia*, ZHANG Zhi-hao, YE Wang-quan, ZHENG Rong-er. The Experimental Research on In-Situ Detection for Dissolved CO2 in
Seawater Based on Tunable Diode Laser Absorption Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1264-1269. |
| [6] |
SU Jing-ming1, 2, 3, ZHAO Min-jie1, ZHOU Hai-jin1, YANG Dong-shang1, 2, HONG Yan3, SI Fu-qi1*. On-Orbit Degradation Monitoring of Environmental Trace Gases Monitoring Instrument Based on Level 0 Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 686-691. |
| [7] |
XI Liang1,2, SI Fu-qi1*, JIANG Yu1, ZHOU Hai-jin1, QIU Xiao-han1, CHANG Zhen1. Ground-Based IDOAS De-Striping by Weighted Unidirectional Variation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 627-633. |
| [8] |
WAN Xiao-ming1, 2, ZENG Wei-bin1, 2, LEI Mei1, 2, CHEN Tong-bin1, 2. Micro-Distribution of Elements and Speciation of Arsenic in the Sporangium of Pteris Vittata[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 478-482. |
| [9] |
WANG Zhao-hui1, ZHAO Yan1, 3, 4*, FENG Chao2. Multi-Wavelength Random Lasing Form Doped Polymer Film With Embedded Multi-Shaped Silver Nanoparticle[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 38-42. |
| [10] |
LIU Guo-hua, LI Qi-hua*, OU Jin-ping, XU Heng, ZHU Peng-cheng, LIU Hao-ran. Passive Spectrum Measurement of HCHO in Chongqing Area Based on MAX-DOAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 243-247. |
| [11] |
ZHONG Yuan, QU Meng-wen, Andy Hsitien Shen*. Comparison of Chemical Composition and Spectroscopy of Purple- Brownish Red Garnet From Zambia, Tanzania and Australia[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 184-190. |
| [12] |
JIANG Cheng1, TANG Gui-qian2*, LI Qi-hua1*, LIU Bao-xian3, WANG Meng2, WANG Yue-si2. Vertical Profile of Aerosol in Spring in Beijing Based on Multi-Axis Differential Optical Absorption Spectroscopy Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 265-271. |
| [13] |
CHENG Liang-xiao1, 2, TAO Jin-hua1*, ZHOU Hai-jin3, YU Chao1, FAN Meng1, WANG Ya-peng4, WANG Zhi-bao5, CHEN Liang-fu1. Evaluations of Environmental Trace Gases Monitoring Instrument (EMI) Level 1 Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3881-3886. |
| [14] |
MA Hong-liang1, 2, ZHENG Jian-jie1, 3, 4, LIU Qiang1, 3*, QIAN Xian-mei1, 3, ZHU Wen-yue1, 3. A Multispectrum Fitting Program Based on Non-Linear Least-Squares Method for Line Parameters：Application to 12CH4[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3887-3891. |
| [15] |
MA Li1, 2, FAN Xin-li1, 2, ZHANG Shuo1, 2, WANG Wei-feng1, 2, WEI Gao-ming1, 2. Research on CH4 Gas Detection and Temperature Correction Based on TDLAS Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(11): 3632-3638. |
|
|
|
|
