光谱学与光谱分析 |
|
|
|
|
|
Study on the Interaction of Ofloxacin and Levofloxacin with DNA |
TONG Chang-lun, ZHANG Xiao-xiang |
Institute of Environmental Science, Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, Zhejiang University, Hangzhou 310029, China |
|
|
Abstract The interaction of ofloxacin (OFLX) and levofloxacin (L-OFLX) with calf thymus DNA(ctDNA)was studied by absorption spectra, fluorescence spectra, fluorescence polarization, and K3Fe(CN)6 quenching tests to show the interaction differences and binding modes. The experimental results of absorption spectra indicated that when ctDNA was added to the solution of OFLX or L-OFLX and its concentration was increased, the hypochromic effects in the absorption spectra of OFLX or L-OFLX were observed, and no changes in its maximum absorption wavelengths were found. Fluorescence spectroscopy was an appropriate method to study the interactions between small molecule ligands and biomacromolecule. From the measurements of emission peaks, transfer efficiency of energy, lifetime and so on, a vast amount of information about the interaction will be given. The experimental results indicated that the fluorescence quenching effects of OFLX and L-OFLX were not initiated by the dynamic collision according to the Stern-Volmer equation, and caused by the static quenching of compounds formation. From the Scatchard equation, its association constants were obtained for OFLX and L-OFLX to be 1.15×105 and 3.75×105 L·mol-1, respectively. On the basis of the absorption spectra and the association constants, it was showed that the interaction between L-OFLX and ctDNA was stronger than that of OFLX and ctDNA. There are three modes for the binding of small molecules to DNA double helix: electrostatic binding, groove binding and intercalative binding. The interaction modes between OFLX, L-OFLX and ctDNA were studied by fluorescence polarization, the K3Fe(CN)6 quenching tests and so on. The results showed that the interaction modes of OFLX and L-OFLX with ctDNA were both groove binding.
|
Received: 2009-02-06
Accepted: 2009-05-08
|
|
Corresponding Authors:
TONG Chang-lun
E-mail: cltong@zju.edu.cn
|
|
[1] HONG Jian-wen, HU Chang-qin, SHENG Long-sheng(洪建文,胡昌勤,盛龙生). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2003, 23(2): 155. [2] TONG Chang-lun, XIANG Guang-hong, HUANG Di-jin, et al(童裳伦,项光宏,黄迪金,等). Chinese Journal of Analytical Chemistry(分析化学),2004,32(5): 619. [3] SHAN Ai-lian, L Yuan, LI Jia-tai(单爱莲,吕 媛,李家泰). The Chinese Journal of Clinical Pharmacology(中国临床药理学杂志),1997,13(2): 115. [4] Tong C L, Hu Z, Liu W P. J. Agric. Food Chem., 2005, 53(16): 6207. [5] Xiang G H, Tong C L, Lin H Z. J. Fluoresc., 2007,17(5): 512. [6] Hu Z, Tong C L. Anal. Chim. Acta, 2007, 587(2): 187. [7] Long E C, Barton J K. Acc. Chem. Res., 1990,23(9):271. [8] Chaires J B, Dattagupta N, Crothers D M. Biochemistry, 1982, 21(17): 3933. [9] LI Jin-huan, AI Shi-yun, SHI Wei-jie, et al(李金焕,艾仕云,时伟杰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(3): 726. [10] Kumar C V, Asuncion E H. J. Am. Chem. Soc., 1993, 115(19): 8547.
|
[1] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[2] |
XU Rong1, AO Dong-mei2*, LI Man-tian1, 2, LIU Sai1, GUO Kun1, HU Ying2, YANG Chun-mei2, XU Chang-qing1. Study on Traditional Chinese Medicine of Lonicera L. Based on Infrared Spectroscopy and Cluster Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3518-3523. |
[3] |
GUO Jing-fang, LIU Li-li*, CHENG Wei-wei, XU Bao-cheng, ZHANG Xiao-dan, YU Ying. Effect of Interaction Between Catechin and Glycosylated Porcine
Hemoglobin on Its Structural and Functional Properties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3615-3621. |
[4] |
ZHANG Xiao-dan1, 2, LIU Li-li1*, YU Ying1, CHENG Wei-wei1, XU Bao-cheng1, HE Jia-liang1, CHEN Shu-xing1, 2. Activation of Epigallocatechin Gallate on Alcohol Dehydrogenase:
Multispectroscopy and Molecular Docking Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3622-3628. |
[5] |
WANG Peng1, GAO Yong-bao1*, KOU Shao-lei1, MEN Qian-ni1, ZHANG Min1, HE Tao1, YAO Wei2, GAO Rui1, GUO Wen-di1, LIU Chang-rui1. Multi-Objective Optimization of AAS Conditions for Determination of Gold Element Based on Gray Correlation Degree-RSM Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3117-3124. |
[6] |
LIU Pan1, 2, 3, DU Mi-fang1*, LI Bin1, LI Jing-bin1, ZENG Lei1, LIU Guo-yuan1, ZHANG Xin-yao1, 4, ZHA Xiao-qin1, 4. Determination of Trace Tellurium Content in Aluminium Alloy by
Inductively Coupled Plasma-Atomic Emission Spectrometry Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3125-3131. |
[7] |
LIU Wen-bo, LIU Jin, HAN Tong-shuai*, GE Qing, LIU Rong. Simulation of the Effect of Dermal Thickness on Non-Invasive Blood Glucose Measurement by Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2699-2704. |
[8] |
YANG Jing1, LI Li1, LIANG Jian-dan1, HUANG Shan1, SU Wei1, WEI Ya-shu2, WEI Liang1*, XIAO Qi1*. Study on the Interaction Mechanism Between Thiosemicarbazide Aryl Ruthenium Complexes and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2761-2767. |
[9] |
WANG Bin1, 2, ZHENG Shao-feng2, GAN Jiu-lin1, LIU Shu3, LI Wei-cai2, YANG Zhong-min1, SONG Wu-yuan4*. Plastic Reference Material (PRM) Combined With Partial Least Square (PLS) in Laser-Induced Breakdown Spectroscopy (LIBS) in the Field of Quantitative Elemental Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2124-2131. |
[10] |
ZHANG Ye-li1, 2, CHENG Jian-wei3, DONG Xiao-ting2, BIAN Liu-jiao2*. Structural Insight Into Interaction Between Imipenem and Metal β-Lactamase SMB-1 by Spectroscopic Analysis and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2287-2293. |
[11] |
LI Chen-xi1, SUN Ze-yu1, 2, ZHAO Yu2*, YIN Li-hui2, CHEN Wen-liang1, 3, LIU Rong1, 3, XU Ke-xin1, 3. The Research Progress of Two-Dimensional Correlation Spectroscopy and Its Application in Protein Substances Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 1993-2001. |
[12] |
HOU Qian-yi1, 2, DONG Zhuang-zhuang1, 2, YUAN Hong-xia1, 2*, LI Qing-shan1, 2*. A Study of the Mechanism of Binding Between Quercetin and CAV-1 Based on Molecular Simulation, Bio-Layer Interferometry and
Multi-Spectroscopy Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 890-896. |
[13] |
WU Lei1, LI Ling-yun2, PENG Yong-zhen1*. Rapid Determination of Trace Elements in Water by Total Reflection
X-Ray Fluorescence Spectrometry Using Direct Sampling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 990-996. |
[14] |
LI Wen, CHEN Yin-yin*, LUO Xue-ke, HE Na. Research on Testing NH3-N and COD in Water Quality Based on
Continuous Spectroscopy Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 254-259. |
[15] |
LI Jin-zhi1, LIU Chang-jin1, 4*, SHE Zhi-yu2, ZHOU Biao2, XIE Zhi-yong2, ZHANG Jun-bing3, JIANG Shen-hua2, 4*. Antiglycation Activity on LDL of Clove Essential Oil and the Interaction of Its Most Abundant Component—Eugenol With Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 324-332. |
|
|
|
|