| 光谱学与光谱分析 |
|
|
|
|
|
| SERS Spectra of Xanthomonas Oryzae pv. Oryzae (Xoo) on Nano Silver Film Prepared by Electrolysis Method |
| KANG Yi-pu1, SI Min-zhen1*, LI Qing-yu2, HUANG Qiong3, LIU Ren-ming1 |
1. Department of Physics and Electron Science, Chuxiong Normal University, Chuxiong 675000, China
2. Fundamental Department of South West Forest College, Kunming 650224, China
3. Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, China |
|
|
|
|
Abstract The nano silver film was prepared by electrolysis method using silver nitrate and polyvinyl alcohol (PVA) in deionized water as the electrolyte, with four glass slides put in the electrolyte and two silver rods dipped into the electrolyte as the anode and cathode. A direct current was applied to the rods, then the four glass slides stayed in the silver colloids. Thus the authors got the nano silver film. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to detect the silver particles in the silver colloids and on the nano silver film. From the SEM we can see that the silver particles on the film formed different layers. In one layer, the distance between two particles was about 100 nm. The samples of Xanthomonas oryzae pv. Oryzae (Xoo) were 7 different kinds of bacterial blight, namely 1-YN1, 2-YN7, 3-YN11, 4-GD414, 5-SCYC6, 6-HEN11 and 7-FWJ. Because the silver particles in the colloids were aggregated on the film, there was large electromagnetic potentiation. So the SERS spectra of Xoo were perfect. The authors used the area analytical method to distinguish the different kinds of Xoo. The silver film prepared by electrolysis was cheap and active, the preparation time of the samples was short, and any normal chemistry lab can make it, which can find excellent application to detecting the Xoo in agriculture. On the other hand, this film is active on biomolecules and bioorganism, which may be a new kind of SERS fundus to explain the creation of the SERS. Further study was under way.
|
|
Received: 2009-01-10
Accepted: 2009-05-20
|
|
|
|
Corresponding Authors:
SI Min-zhen
E-mail: siminzhen@hotmail.com
|
|
[1] Moskovits M. Rev. Mod. Phys., 1985, 57: 783.
[2] Xiaofang Z, Yan F, Pengxiang Z. Spectrochimica Acta Part A, 2007, 67: 122.
[3] Yantu Z, Zhujun Z, Yonghua S. Journal of Chromatography A, 2006, 1129: 34.
[4] Le Ru E C, Blackie E, Meyer M, et al. J. Phys. Chem., 2007, 111: 37.
[5] Choi S H, Wang D, Williams J R, et al. Applied Surface Science, 2007, 253: 5411.
[6] Yakutik I M, Shevchenko G P, Rakhmanov S K. Colloids and Surface, 2004, 242: 175.
[7] Xiaomiao H, Yan F. Colloid and Interface Science, 2007, 316: 19.
[8] Ratna T, Richard J C Brown, Martin J T Milton. Journal of Raman Spectrum, 2007, 38: 1469.
[9] SI Min-zhen, WU Rong-guo, ZHANG Peng-xiang(司民真, 武荣国, 张鹏翔). Acta Photonica Sinica(光子学报), 1999, 28: 839.
[10] Wang J J, Saito Y, Batchelder D N, et al. App. Phy. Lett., 2005, 86: 263111.
[11] Qiu T, Wu X L, Shen J C, et al. App. Phy. Lett., 2006, 89: 131914.
[12] Chu L Y, Shun Y P, Wang C C, et al. Phytochemistry, 2008, 69: 1989.
[13] Cho H J, Park Y J, Noh T H, et al. Microbial Pathogenesis, 2008, 44: 473.
[14] Moon W J, Cho J Y, Chae Y K. Protein Expression and Purification, 2008, 10: 1016.
[15] XU Zhi-gang, SUN Qi-ming, LIU Feng-quan, et al(许志刚，孙启明，刘凤全，等). Chinese Journal of Rice Science(中国水稻科学)，2004，18(5)：469.
[16] JI Guang-hai, XIA Xian-ren, XIAO Lu-ting, et al(姬广海, 夏贤仁, 肖鲁婷, 等). Journal of Yunnan Agricultural University(云南农业大学学报), 2004, 19(5): 541.
[17] YU Jing, ZHOU Yong-li, CANG Jing, et al(于 晶，周永力，苍 晶，等). Acta Agronomica Sinica(作物学报)，2006, 32: 1611.
[18] LIU Yong, MO Xiao-han, YU Qing, et al(刘 勇，莫笑晗，余 清，等). Acta Phytopathologica Sinica(植物病理学报), 2006, 36(4): 310.
[19] SI Min-zhen, FANG Yan, PENG Jia-lin, et al(司民真，方 炎，彭家林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(5): 948.
[20] Ranjith Premasiri W, Moir D T, Ziegler L D. Chemical and Biological Sensing Ⅵ, 2005, 5795: 19.
[21] Jarvis R M, Goodacre R. Anal. Chem., 2004, 76: 40.
[22] Rothschild K J, Andrew J R, De Grip W J, et al. Science, 1976, 191: 1176.
[23] Susi H, Sampugna J, Hampson J W, et al. Biochemistry, 1979, 18: 297.
|
| [1] |
LIU Xin-peng1, SUN Xiang-hong2, QIN Yu-hua1*, ZHANG Min1, GONG Hui-li3. Research on t-SNE Similarity Measurement Method Based on Wasserstein Divergence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3806-3812. |
| [2] |
ZHAO Yu-wen1, ZHANG Ze-shuai1, ZHU Xiao-ying1, WANG Hai-xia1, 2*, LI Zheng1, 2, LU Hong-wei3, XI Meng3. Application Strategies of Surface-Enhanced Raman Spectroscopy in Simultaneous Detection of Multiple Pathogens[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2012-2018. |
| [3] |
LI Jia-jia, XU Da-peng *, WANG Zi-xiong, ZHANG Tong. Research Progress on Enhancement Mechanism of Surface-Enhanced Raman Scattering of Nanomaterials[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1340-1350. |
| [4] |
HUANG Hui1, 2, TIAN Yi2, ZHANG Meng-die1, 2, XU Tao-ran2, MU Da1*, CHEN Pei-pei2, 3*, CHU Wei-guo2, 3*. Design and Batchable Fabrication of High Performance 3D Nanostructure SERS Chips and Their Applications to Trace Mercury Ions Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3782-3790. |
| [5] |
QIU Meng-qing1, 2, XU Qing-shan1*, ZHENG Shou-guo1*, WENG Shi-zhuang3. Research Progress of Surface-Enhanced Raman Spectroscopy in Pesticide Residue Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(11): 3339-3346. |
| [6] |
LI Guang-mao, QIAO Sheng-ya, ZHU Chen, ZHENG Fu-li, YANG Sen, CAI Han-xian. Preparation and Application of Micro-Nano Structure SERS Substrate Based on Copper Mesh Displacement Reaction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3166-3171. |
| [7] |
GUI Bo1, 2, YANG Yu-dong1, ZHAO Qian1, 2, SHI Meng1, MAO Hai-yang1, 3*, WANG Wei-bing1, CHEN Da-peng1, 3. A SERS Substrate for On-Site Detection of Trace Pesticide Molecules Based on Parahydrophobic Nanostructures[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(08): 2499-2504. |
| [8] |
LI Ling1,2, HE Xin-yu1,2, LI Shi-fang1,2, GE Chuang3*, XU Yi1,2,4*. Research Progress in Identification and Detection of Fungi Based on SERS Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1661-1668. |
| [9] |
TIAN Hui-yan1，LIU Yu1, HUANG Jiao-qi1, XIE Feng-xin1, HUANG Guo-rong1, LIAO Pu1, FU Wei-ling1, ZHANG Yang2*. Research Progress and Application of Surface-Enhanced Raman Scattering Technique in Nucleic Acid Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3021-3028. |
| [10] |
ZHANG Xu, XIN Kun, SHI Xiao-feng*, MA Jun*. Surface-Enhanced Raman Scattering with Au Nanoparticles Optically Trapped by a Silicon-Based Micro-Nano Structure Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(07): 2116-2121. |
| [11] |
ZHAO Qian1,2, YANG Yu-dong1, GUI Bo1,2, MAO Hai-yang1,2,3*, LI Rui-rui1, CHEN Da-peng1,2,3. Surface-Enhanced Raman Scattering Transparent Devices Based on Nanocone Forests[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1168-1173. |
| [12] |
CHEN Shan-jun1*, FAN Jian1, LUO Zhi-neng1, CHEN Yan1, 2, LI Song1, ZHANG Wei-bin1, LU Nian1, WEI Jian-jun3. Theoretical and Experimental Study of Surface Enhanced Raman Spectroscopy of Caffeic Acid Molecules[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(06): 1763-1767. |
| [13] |
YANG Huan-di, LIN Xiang*, LIU Yuan-lan, ZHAO Hai-yan, WANG Li*. Preparation of Three-Dimensional Hotpot SERS Substrate with Sliver Nanocubes and Its Application in Detection of Pesticide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 99-103. |
| [14] |
LIU Yan-de, ZHANG Yu-xiang, WANG Hai-yang. Quantitative Detection of Mixing Pesticide Residues on Navel Orange Based on Surface-Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 123-127. |
| [15] |
LIANG Ai-hui, WANG Yao-hui, OUYANG Hui-xiang, WEN Gui-qing, ZHANG Xing-hui, JIANG Zhi-liang*. Vitoria Blue B SERS Molecular Probe Detection of Escherichia coli in the Silver Nanorod/AgCl Sol Substrate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(11): 3446-3448. |
|
|
|
|
