| 光谱学与光谱分析 |
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| X-Ray Microanalysis of the Activity of Immobilized Glucose Oxidase on Enzyme Electrode |
| LI Tong, YAO Zi-hua*, QIU Man-de, AN Wei, SHI Da-gang |
| College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China |
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Abstract The localization of activity of glucose oxidase (GOD) on the pussian blue modified electrode by SiO2 sol-gel was studied by X-ray microanalysis.Glucose and Ce(NO3)3 served as substrate and capture respectively.Substrate was catalysed by immobilized GOD to produce H2O2，and the H2O2 was captured by Ce(NO3)3 to form precipitate.The precipitation deposited the active site of immobilized GOD on the enzyme electrode.The X-ray microanalysis showed that the distribution of active enzyme on the surface of the enzyme electrode was uniform, and the GOD immobilized maintained high activity.The X-ray analysis exhibited that the properties of the enzyme electrode depended on the distribution of enzyme activity on its surface from the view of microstructure, and the developed GOD electrode was of high sensitivity and excellent stability.The results agreed with the results of the electrochemical study.
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Received: 2004-03-01
Accepted: 2004-07-06
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Corresponding Authors:
YAO Zi-hua
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| Cite this article: |
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LI Tong,YAO Zi-hua,QIU Man-de, et al. X-Ray Microanalysis of the Activity of Immobilized Glucose Oxidase on Enzyme Electrode [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1151-1154.
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https://www.gpxygpfx.com/EN/Y2005/V25/I07/1151 |
[1] LI Tong, YAO Zi-hua(李 彤，姚子华).Chinese Journal of Analytical Chemistry(分析化学), 2004，32(2)： 237.
[2] Ivanildo Luiz de Mattos, Lilia V Lukachova, Lo Gorton, Thomas Laurell, Arkady A Karyakin.Talanta, 2001, 54: 963.
[3] Manoj Ku Ram, Paolo Bertoncello, H Ding, Sergio Paddeu，Claudio Nicolini.Biosensors & Bioelectronics.2001, 16: 849.
[4] WU Xia-qin, CAO Da-wei, JIANG Hui-wen(吴霞琴，曹大为，蒋慧雯).J.of Shanghai Teachers University(Natural Sciences)(上海师范大学学报·自然科学版)，1994，23(2): 159.
[5] HUANG Yong-zhang, YAO Zi-hua, WANG Gui-hua, et al(黄永章，姚子华，王桂华，等).Chinese Iournal of Analytical Chemistry(分析化学), 2001，29(12): 1434.
[6] DING Liang, YAO Zi-hua, LI Tong, QIU Man-de(丁 良，姚子华，李 彤，仇满德).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003，23(2): 400.
[7] YAO Zi-hua, HUANG Yong-zhang, QIU Man-de(姚子华，黄永章，仇满德).Journal of Chinese Electron Microscopy Society(电子显微学报)，2001, 20(1): 27.
[8] Ogawa Kazuo editor(Japan), Translated by ZHONG Ci-sheng(小川和郎(日)主编，钟慈声主译).Techniques of Enzyme Cytochemistry and Histochemistry(酶组织和细胞化学技术), Shanghai： Shanghai Medical University Press(上海： 上海医科大学出版社)，1989.
[9] Richard T Briggs, David B Drath, Manfred L Karnovsky, Morris J Karnovsky.The Journal of Cell Biology.1975, 67: 566.
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