| 光谱学与光谱分析 |
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| Interaction of Quercetin and Bovine Serum Albumin |
| WANG Chun, WU Qiu-hua, WANG Zhi*, CHEN Da-gang |
| College of Sciences, Agricultural University of Hebei, Baoding 071001, China |
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Abstract The interaction of quercetin and bovine serum albumin (BSA) was investigated using fluorescence spectroscopy (FS) and ultraviolet spectroscopy (UV). The apparent binding constants (KA) between quercetin and BSA were 2.8×108(26 ℃) and 3.1×108(36 ℃), and the binding sites (n) were 1.7±0.02. According to the Frster theory of non-radiation energy transfer, the binding distances (r) were also obtained. The experimental results showed that the quercetin could be inserted into the BSA, quenching the inner fluorescence by forming the quercetin-BSA complex. It was found that both static quenching and non-radiation energy transfer were the main reasons for the fluorescence quenching. The process of binding was a spontaneous molecular interactio1n in which entropy increased while Gibbs free energy decreased, indicating that the interaction of quercetin and BSA was driven mainly by hydrophobic force.
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Received: 2005-12-10
Accepted: 2006-03-20
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Corresponding Authors:
WANG Zhi
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