| 光谱学与光谱分析 |
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| FTIR Characterization of the Secondary Structure of a Staphylokinase Variant (K35R) Encapsulated within PLGA Microspheres |
| HE Jin-tian1,2, WANG Gai-zhen3, SONG Hou-yan1,2 |
1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China
2. Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, China
3. College Laboratory Environmental Science and Engineering, Hebei University of Science and Technology Shijiazhuang 050018, China |
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Abstract The secondary structure of a staphylokinase variant (K35R, DGR) encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres was quantitatively examined by Fourier transform infrared (FTIR) spectroscopy. Resolution enhancement technique and Fourier deconvolution were combined with band with curve-fitting procedures to quantitate the spectral information from the amide Ⅰ bands. Nine component bands were found under the broad, nearly featureless amide Ⅰ bands and assigned to α-helix, β-sheet, turn and irregular(random) structures. The changes of bands at 1 651 and 1 623 cm-1 after encapsulation were discussed.
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Received: 2005-05-23
Accepted: 2005-08-24
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Corresponding Authors:
HE Jin-tian
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| Cite this article: |
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HE Jin-tian,WANG Gai-zhen,SONG Hou-yan. FTIR Characterization of the Secondary Structure of a Staphylokinase Variant (K35R) Encapsulated within PLGA Microspheres[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1209-1212.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I07/1209 |
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