| 光谱学与光谱分析 |
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| Application of L-Cysteine-Capped Nano-ZnS as a Fluorescence Probe to the Determination of Proteins in Human Serum |
| ZHAO Dan-hua1, LI Yong-xin2,ZHU Chang-qing2 |
1. Chemistry Department, Huaibei Coal Normal College, Huaibei 235000, China
2. College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China |
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Abstract Nanometer-sized L-cysteine-capped ZnS particles as a fluorescence probe were synthesized, and the effect of proteins on the fluorescence intensity has been investigated. With Δλ=190 nm, the maximum and constant synchronous fluorescence enhancement was produced at 268.0 nm and pH 5.12 in the presence of proteins. A highly sensitive synchronous fluorescence method for the rapid determination of proteins was developed. Under optimum conditions, the calibration curve was linear over the range 0.05-6.0 μg·mL-1 for human serum albumin (HSA), and 0.05-8.0 μg·mL-1 for γ-globulin(γ-G), respectively. The relative standard deviations of seven measurements were 1.90% for 1.0 μg·mL-1 HSA, and 1.65% for 1.0 μg mL-1 γ-G.
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Received: 2005-04-08
Accepted: 2005-08-18
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Corresponding Authors:
ZHAO Dan-hua
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| Cite this article: |
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ZHAO Dan-hua,LI Yong-xin,ZHU Chang-qing. Application of L-Cysteine-Capped Nano-ZnS as a Fluorescence Probe to the Determination of Proteins in Human Serum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1318-1321.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I07/1318 |
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