光谱学与光谱分析 |
|
|
|
|
|
Plasma Glutathione of Patients with Coronary Heart Disease Measured by Fluorospectrophotometer |
HUANG Yan-sheng1, ZHI Yan-fang1, KONG Shen-yan2, WANG Qiu-lin1, XU Bo-shi3 , WANG Shu-ren1* |
1. Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China 2. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China 3. Center Laboratory, Henan Provincial People’s Hospital, Zhengzhou 450003, China |
|
|
Abstract The standard samples of reduction form glutathione (GSH) and oxidization form glutathione (GSSG) were scanned with full-wavelength range to determine the excitation wavelength λex 334.4 nm,the emission wavelength λem 424 nm, and the spectral bandwidth 5 nm respectively. Phosphate buffer saline (PBS) of pH 8.3 served as buffer solution. GSH was incubated for 30 min with 100 μL o-phthaldehyde (OPA) of 10 mmol·L-1 methyl alcohol solution for derivatization, and then fluorescence intensities were measured. With standard glutathione concentration being independent variable and fluorescence intensity being dependent variable,the linear equations for GSH and GSSG were deduced: YGSH=6.9+8.6X (r2=0.994) and YGSSG=6.2+17.2X (r2=0.999). Standard curves were done hereby. The plasma glutathione of three groups was then measured, and GSH/GSSG redox potential was calculated according to Nernst equation. The results indicated that, from normal control group to prophase coronary heart disease group, then to coronary heart disease group, the GSH and GSH/GSSG ratio gradually reduced, GSSG and GSH/GSSG redox potential gradually increased (more positive) (all P<0.05), and the redox potential shifted to oxidization direction along with the development of coronary heart disease. This fluorospectrophotometry method showed simple operation, and fine precision and accuracy.
|
Received: 2005-11-16
Accepted: 2006-02-08
|
|
Corresponding Authors:
WANG Shu-ren
|
|
Cite this article: |
HUANG Yan-sheng,ZHI Yan-fang,KONG Shen-yan, et al. Plasma Glutathione of Patients with Coronary Heart Disease Measured by Fluorospectrophotometer [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 936-940.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2006/V26/I05/936 |
[1] Marczin N, Bundy R E, Hoare G S. Coron. Artery Dis.,2003, 14(2): 123. [2] Jones D P,Vino C Mody Jr, et al. Free Radic. Biol. Med., 2002, 33(12): 1290. [3] SUN Yue, MA Li, WANG Huai-you, et al(孙 悦, 马 骊, 王怀友, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002,22(4): 637. [4] Cereser C, Guichard J, Drai J, et al. Journal of Chromatography B, 2001, 752(2): 123. [5] Cotgreave I A, Moldéus P. J. Biochem. Biophys. Methods, 1986, 13(4): 231. [6] Chong C Y, Huxtable R J. Journal of Chromatography B, 1995, 672(2): 217. [7] Morineau G, Azoulay M, Frappier F. Journal of Chromatography. A, 1989, 467(1): 209. [8] Munday R. Free Radic. Biol. Med., 1989, (6)7: 659. |
[1] |
ZHAO Jing1, MA Bei1, LIU Ming2*, LIU Zhen-zhen1, LI Gang3, LI Zhe4, WANG Yi-min1*. Study on Screening of Hypertension Complicated With Coronary Heart Disease Based on Tongue Hyperspectral Imaging[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 512-516. |
[2] |
HOU Jun-feng1, LI Xin-xia2, SHEN Xue-ru1, Miliban Huojia1, GUAN Ming1* . Determination of H2S in Rat Intestinal Perfusion Solution Based on Fluorescence Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(08): 2217-2220. |
[3] |
LU Kui1,2, CHENG Hong-li1, MA Li1, ZHAO Dong-xin1, YANG Liang-ru1, ZHAO Yu-fen3 . Study on the Interaction of L-Cysteine Dipeptide with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 146-149. |
[4] |
SHEN Xiao-shu, GUO Lei*, GU Ming-song, FENG Jian-lin, XIE Jian-wei . Rapid Determination of Tetrodotoxin Using Microwave Assisted Alkaline Hydrolysis and Spectrofluorometric Analysis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2264-2267. |
[5] |
HE Hua1,WANG Ling-li1,LIU Xu-hui2,JIAO Qing-cai3,CHUONG Pham-Huy4,WANG Guang-ji1* . Fluorescence Characteristic of Terbium-Ciprofloxacin Complex and Its Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1516-1519. |
[6] |
HE Hua1,YE Hai-ying1,DAI Li1,JIAO Qing-cai2,CHUONG Pham-Huy3 . Study on the Interaction of Lomefloxacin Terbium Complex and Bovine Serum Albumin by Fluorescence Spectra [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 480-483. |
[7] |
XU Li-ying, YU Wen-qing. The Fluorescence Characteristics of Norfloxacin and Its Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1615-1617. |
[8] |
KAI Xiao-ming. The Pattern of Levenberg-Marquardt Backpropagation Algorithm for Recognition of Coronary Heart Disease Patients Based on Microamount of Elements in Human Blood [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1412-1414. |
[9] |
DU Li-ming, WANG Jing-ping, WANG Cai-xia . The Fluorescence Characteristics of Micelle Inclusion of Pefloxacin and Its Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 855-857. |
[10] |
GUO Liang-qia, XIE Zeng-hong*, LIN Xu-cong, KE Zi-hou, CHEN Guo-nan . Direct Fluorophotometric and Flow-Injection Fluorophotometric Methods for the Determination of Trace Ammonia [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 851-854. |
|
|
|
|