| 光谱学与光谱分析 |
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| Rapid Determination of Tetrodotoxin Using Microwave Assisted Alkaline Hydrolysis and Spectrofluorometric Analysis |
| SHEN Xiao-shu, GUO Lei*, GU Ming-song, FENG Jian-lin, XIE Jian-wei |
| Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China |
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Abstract To develop a rapid, simple and sensitive method for determining the concentration of tetrodotoxin(TTX), TTX was hydrolyzed in the strong alkali solution of water mixed with isopropanol. The concentration of TTX can be indirectly analyzed by the fluorescence signal of its alkaline hydrolysis product which can be enhanced by the microwave method. The maximum excitation and emission wavelengths of the alkaline hydrolysis product of tetrodotoxin were 380 and 496 nm, respectively. The linear range of the calibration curve was 0.1-10 μmol·L-1 with r=0.9991. The limit of detection was 0.05 μmol·L-1,which was twenty times lower than before. A rapid, highly sensitive and accurate method was thus established. It can be used as a quantitative method for detecting TTX.
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Received: 2005-09-08
Accepted: 2006-01-02
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Corresponding Authors:
GUO Lei
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| Cite this article: |
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SHEN Xiao-shu,GUO Lei,GU Ming-song, et al. Rapid Determination of Tetrodotoxin Using Microwave Assisted Alkaline Hydrolysis and Spectrofluorometric Analysis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2264-2267.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I12/2264 |
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