| 光谱学与光谱分析 |
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| Study on the Spectra of Cal-Red-Cu(Ⅱ) Complex with Bovine Serum Albumin and Its Analytical Application |
| CHEN Dan-dan1,ZHANG Tao1,REN Li-ping1*,SHI Deng-rong2,YU Jin-lian2 |
| 1. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100094, China2. Department of Environmental Science and Engineering, College of Tourism, Shanghai Normal University, Shanghai 200234, China |
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Abstract The Resonance light scattering (RLS) spectra, fluorescence spectra, and absorption spectra of Cal-Red-Cu(Ⅱ) metal complex with bovine serum albumin(BSA) were studied. As the Cal-Red-Cu(Ⅱ)-BSA complex formed, the RLS spectra and fluorescence spectra enhanced, but the absorption spectra weakened and the absorption peak at 594 nm disappeared. At pH 5.65-5.75，the enhanced RLS of Cal-Red-Cu(Ⅱ) metal complex with BSA system was characterized by the maximum scattering at 317 nm, and the RLS intensity is proportional to the concentration of BSA. Under optimal conditions, the linear range of calibration was 0.75-10 μg·mL-1,the linear regression equation was I=150.88+201.48c(BSA，μg·mL-1)with r=0.997 3, and the limit of detection was 5.62×10-2 μg·mL-1. The method was applied to the determination of BSA in synthetic samples satisfactorily. A mechanism of electrostatic force was suggested for the interaction between BSA and Cal-Red-Cu(Ⅱ).
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Received: 2005-09-28
Accepted: 2005-12-18
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Corresponding Authors:
REN Li-ping
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| Cite this article: |
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CHEN Dan-dan,ZHANG Tao,REN Li-ping, et al. Study on the Spectra of Cal-Red-Cu(Ⅱ) Complex with Bovine Serum Albumin and Its Analytical Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 686-689.
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http://www.gpxygpfx.com/EN/Y2006/V26/I04/686 |
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