| 光谱学与光谱分析 |
|
|
|
|
|
| Microemulsion Sensitized Fluorophotometric Determination of Protein with Probe of Phenylfluorone-Molybdenum(Ⅵ) Complex |
| WEI Qin, LI Yan, DUAN Cai-hong, GUO Gui-xiang, DU Bin |
| School of Chemistry and Chemical Engineering, Jinan University, Jinan 250022, China |
|
|
|
|
Abstract The fluorescence spectral behavior of interaction of phenylfluorone(PF)-Mo(Ⅵ) and protein was investigated in Triton X-100 microemulsion medium at pH 2.0. A novel method for the determination of protein using phenylfluorone (PF)-Mo(Ⅵ) as a fluorescence spectrum probe was developed. Excitation and emission wavelengths are 465 and 525 nm, respectively. The effective factors and the optimum conditions have been studied, and the reducing value of fluorescence intensity is in proportion to the concentration of proteins in the range 0-6.00 μg·L-1 for bovine serum albumin, 0-4.00 μg·L-1 for human serum albumin, 0-5.00 μg·L-1 for ovalbumin, and 0-4.00 μg·L-1 for lysozyme. The Triton X-100 microemulsion was efficiently used to enhance the sensibility and stability of the system, and the limits of detection are 5.4, 5.2, 1.5 and 8.2 ng·L-1, respectively. Most of foreign substances do not interfere with the determination, and this method has good selectivity and high sensitivity. It has been applied to the determination of proteins in the urine samples with satisfactory results.
|
|
Received: 2004-10-29
Accepted: 2005-01-28
|
|
|
|
Corresponding Authors:
WEI Qin
|
|
| Cite this article: |
|
WEI Qin,LI Yan,DUAN Cai-hong, et al. Microemulsion Sensitized Fluorophotometric Determination of Protein with Probe of Phenylfluorone-Molybdenum(Ⅵ) Complex [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 109-112.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2006/V26/I01/109 |
[1] XIAO Guo-rong, HUANG Xuan-zhong(肖国荣, 黄选忠). Chinese Journal of Analysis Laboratory(分析试验室), 2001, 20(3): 63.
[2] TANG Gui-na, WEI Wei, WANG Bao-ning(汤桂那, 魏 巍, 王保宁). Chinese Journal of Analytical Chemistry(分析化学), 1993, 21(7): 831.
[3] SHEN Han-xi, LIU Liu-zhan, LIU Yu-guo(沈含熙, 刘六战, 刘玉国). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(3): 444.
[4] CHEN Hong-qi(陈鸿琪). Journal of Northwest Normal University(Natural Science)(西北师范大学学报·自然科学版)，2000，36(3): 53.
[5] DU Bin，LI Yan，ZHANG Hui，WU Dan，WEI Qin，LI Zai-jun(杜 斌, 李 燕, 张 慧, 吴 丹, 魏 琴, 李在均). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005，25(7)：1110.
[6] Guo Zhong-xian, Shen Han-xi. Analytica Chimica Acta, 2000, 408(1-2): 177.
|
| [1] |
CHU Bing-quan1, 2, LI Cheng-feng1, DING Li3, GUO Zheng-yan1, WANG Shi-yu1, SUN Wei-jie1, JIN Wei-yi1, HE Yong2*. Nondestructive and Rapid Determination of Carbohydrate and Protein in T. obliquus Based on Hyperspectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3732-3741. |
| [2] |
CHENG Hong1, YAN Ding-ce1*, WU Li-qing2, XU Jun3. Spectral Magnitude Uncertainty in Measurement of Protein Circular
Dichroism Spectra—An Empirical Study on Cytochrome C[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3105-3110. |
| [3] |
ZHANG Peng1, 3, YANG Yi-fan1, WANG Hui1, TU Zong-cai1, 2, SHA Xiao-mei2, HU Yue-ming1*. A Review of Structural Characterization and Detection Methods of Glycated Proteins in Food Systems[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2667-2673. |
| [4] |
CHEN Rui1, WANG Xue1, 2*, WANG Zi-wen1, QU Hao1, MA Tie-min1, CHEN Zheng-guang1, GAO Rui3. Wavelength Selection Method of Near-Infrared Spectrum Based on
Random Forest Feature Importance and Interval Partial
Least Square Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1043-1050. |
| [5] |
LIAN Xiao-qin1, 2, CHEN Qun1, 2, TANG Shen-miao1, 2, WU Jing-zhu1, 2, WU Ye-lan1, 2, GAO Chao1, 2. Quantitative Analysis Method of Key Nutrients in Lanzhou Lily Based on NIR and SOM-RBF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2025-2032. |
| [6] |
LIU Mei-chen, XUE He-ru*, LIU Jiang-ping, DAI Rong-rong, HU Peng-wei, HUANG Qing, JIANG Xin-hua. Hyperspectral Analysis of Milk Protein Content Using SVM Optimized by Sparrow Search Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1601-1606. |
| [7] |
CHEN Hua-zhou1,2, XU Li-li3, QIAO Han-li1,2, HONG Shao-yong4*. Latent Variable Machine Learning Methods Applied for NIR Quantitative Analysis of Coffee[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1441-1445. |
| [8] |
FAN Nai-yun, LIU Gui-shan*, ZHANG Jing-jing, ZHANG Chong, YUAN Rui-rui, BAN Jing-jing. Hyperspectral Model Optimization for Protein of Tan Mutton Based on Box-Behnken[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 918-923. |
| [9] |
WANG Cheng-ke, ZHANG Ze-xiang, HUANG Xiao-wei*, ZOU Xiao-bo*, LI Zhi-huang, SHI Ji-yong. Detection of Component Content Changes During Tofu Formation Based on Hyperspectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3549-3555. |
| [10] |
ZHU Li-wei1, ZHOU Yan1, CAI Fang1, DENG Jiao1, HUANG Juan1, ZHANG Xiao-na1, ZHANG Jin-ge2, CHEN Qing-fu1*. Quantitative Analysis of Perennial Buckwheat Leaves Protein and GABA Using Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2421-2426. |
| [11] |
LÜ Fei, ZHANG Jing, CHEN Xin-lu, LIU Jian-hua, DING Yu-ting*. Spectroscopic Method Combined with Molecular Dynamics Simulation to Study the Action Mechanism of Ozone on Myoglobin Structure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3115-3121. |
| [12] |
XU Kun1,2,3, WANG Ju-lin1,2,3*. Micro-Destructive and Rapid Chemical Identification of the Protein Binders in Colors Layers of Paintings by ATR-FTIR and Principal Component Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2299-2306. |
| [13] |
JIANG Lu-lu1, WEI Xuan2, XIE Chuan-qi3*, HE Yong4*. Non-Destructive Determination of Growth Quality Indicators of Spirulina sp. Using Vis/NIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2493-2497. |
| [14] |
WANG Zhi-hui1, LONG Dan-dan2, LI Zi-juan2, YANG Ya-fei3, YAN Jing-hui2*, ZOU Ming-qiang4,5*. One-Pot Microemulsion Synthesis of NaYF4∶Yb3+, Ho3+@Au[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1793-1797. |
| [15] |
CHEN Lei1, 2, LIU Mao-mao2, ZHANG Yong-jun2*, WANG Ya-xin2, HAN Xiao-xia3, ZHAO Bing3. Indirect Protein Detection by Versatile SERS Sensors[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(10): 3094-3098. |
|
|
|
|
