| 光谱学与光谱分析 |
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| Studies on the Interaction between RNA with Methyl Violet and Determination of RNA by Spectrophotometry |
| SI Wen-hui1, ZI Yan-qin2 |
1. Suzhou Agriculture College, Suzhou 215008, China
2. Department of Chemistry, Huaibei Coal College, Huaibei 235000, China |
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Abstract An analytical method for the determination of ribonucleic acid was established by spectrophotometry. At maxium absorption wavelength for methyl violet in the B-R buffer solution, and under the best conditions, the degree of decrease of the absorbance was linear with the amount of ribonucleic Acid. It was a new and preferable approach for the determination of ribonucleic Acids. The method with the linearity range was 1.0 to 8.0 μg·mL-1 and the detection limit was 0.52 μg·mL-1 , and the correlation coeffient was 0.999 9. This method was simple, rapid, and selective. So it was satisfactory to the application for the determination of ribonucleic Acid. The reaction mechanism was electrostatic interaction to make molecular association of RNA with methyl violet, to go on antiion permutation and bonded reaction of concert.
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Received: 2005-03-16
Accepted: 2005-06-26
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Corresponding Authors:
SI Wen-hui
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| Cite this article: |
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SI Wen-hui,ZI Yan-qin. Studies on the Interaction between RNA with Methyl Violet and Determination of RNA by Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(11): 1846-1849.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I11/1846 |
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