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| FTIR Study on the Effect of DangGuiTongFengFang on Hyperuricemia Nephropathy in Mice |
| LIU Bi-wang1, LU Rong-rong2, CAO Yue2, WANG Xiu-wen3, ZHAO Huan4, HAO Miao3, MA Xiao-xia3, MA Yan-miao2, WANG Yong-hui2 |
1. FUSHAN College, Shanxi University of Chinese Medicine, Yuci 030619，China
2. Basic Medicine College, Shanxi University of Chinese Medicine, Yuci 030619，China
3. College of TCM and Food Engineering, Shanxi University of Chinese Medicine, Yuci 030619，China
4. Pharmacy Department, Affiliated Hospital of Shanxi University of Chinese Medicine, Taiyuan 030024，China
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Abstract The Fourier transform infrared spectrometer (FTIR) is an effective method to identify and analyze the structure of substances. It can be used to characterize Quantitative analysis samples and is widely used in many fields, such as medicine and the chemical industry. FTIR, as a simple, practical, accurate and low-cost identification method, has been widely used in drug testing, but its application in pharmacological research is very limited. There are only reports on the study of various kinds of tumor tissues by infrared spectrum analysis. Hyperuricemia nephropathy is a condition in which a high uric acid load exceeds the ability of the kidneys to clear the elevated level of serum uric acid. The uric acid crystals are deposited in collecting ducts, the renal pelvis and the urethra. Therefore, it is feasible to use FTIR to evaluate the Hyperuricemia nephropathy model and evaluate the efficacy of drugs. To explore the feasibility of FTIR in evaluating the Hyperuricemia nephropathy model and the therapeutic effect of DangGuiTongFengFang, 60 km mice were randomly divided into 6 groups, the Rats in the normal control group, model group, positive control group and the low, middle and high groups of the Chinese angelica gouty prescription were treated with potassium oxonateto make the model of Hyperuricemia nephropathy, serum UA, urine UA, serum CRE, urine CRE, serum IL-1β, renal IL-1β and FTIR spectra were detected. The results showed that the indexes of the model group were significantly different from those of the normal group (p<0.05), and the serum levels of UA, CRE, IL-1β and renal IL-1β in the high-dose group were also significantly higher than those in the model group (p<0.05). The pathological changes of the kidney in the high-dose group were improved, with no inflammatory cell infiltration and no tubular type in the lumen, indicating that the high-dose group has a protective effect on the kidney injury. The FTIR spectra of the model group were lower than that of the normal group. The FTIR spectra of the positive control group and the high-dose group of DangGuiTongFengFang were close to that of the normal group. It is further indicated that DangGuiTongFengFang has a protective effect on renal injury. The study shows that FTIR can be used as a simple, quick, flexible and economical method to evaluate the animal model and the therapeutic effect of drugs. It also provides a significant technical means for the Hyperuricemia nephropathy model and the screening of effective drugs. This experiment extends the application range of the FTIR detection method.
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Received: 2022-07-27
Accepted: 2022-11-27
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