血红蛋白与铜(Ⅱ)-茜素红S络合物相互作用的研究

Abstract
References
Related Citation (15)

Download: PDF (772 KB)
Export: BibTeX | EndNote (RIS)

Abstract The reaction of hemoglobin (Hb) with copper(Ⅱ)-Alizarin red S (ARS) complex was studied in H₃PO₄-KH₂PO₄ buffer solution (pH 4.2) by ultraviolet-visible spectrophotometry. The results show that the interaction of Hb and Cu(Ⅱ)-ARS complex produces red ionic association complex with its maximum absorption peak at 537 nm. At the maximum absorption, the composition of the complex was determined to be n_Hb∶n_Cu(Ⅱ)∶n_ARS=1∶4∶8, and the apparent molar absorptivity was 1.52×10⁵ L·mol^-1·cm^-1. The concentration of Hb is linear with the absorbency in the range of 1.0×10^-7-2.0×10^-6 mol·L^-1 and the regression equation was established as A=0.026 9+151 675ｃ(mol·L^-1) with the coefficient r=0.997 2. The effects of solution acidity, reagent amount, reaction time, temperature, ionic strength and the added surfactant were examined on the formation of the Hb-Cu(Ⅱ)-ARS complex. A preliminary investigation was carried out to elucidate the reaction mechanism, and it could be concluded that the Hb and Cu(Ⅱ)-ARS complex are combined mainly by electrostatic attraction. Further investigation was also undertaken to find out the effects of common amino acids and metallic ions on the formation of Hb-Cu(Ⅱ)-ARS complex.

Key words：Hemoglobin;Copper(Ⅱ)-alizarin red S complex;Ultraviolet-visible spectrophotometry

Received: 2006-12-09 Accepted: 2007-04-24

ZTFLH:

O657.3

Corresponding Authors: WU Xiao-hua E-mail: sky61@zjnu.cn

Cite this article:

WU Xiao-hua,MIAO Ji-gen,MIAO Yu-qing, et al. Study on the Interaction of Hemoglobin and Cu(Ⅱ)-ARS Complex[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(06): 1168-1171.

URL:

https://www.gpxygpfx.com/EN/Y2007/V27/I06/1168

[1] Congdon R W，Muth G W，Splittgerber A G. Analytical Biochemistry，1993，213：407.
[2] Pesavento M，Profumo A. Talanta，1991，38：1099.
[3] Bradford M M. Analytical Biochemistry，1976，72：248.
[4] YU Ying，ZHOU Zhen-tao(俞英，周震涛). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(4)：628.
[5] WEI Qin，LI Yan，YU Hai-qin，et al(魏琴，李燕，于海琴，等). Chinese Journal of Analytical Chemistry(分析化学)，2005，33(8)：1175.
[6] WEI Qin，LI Yan，DUAN Cai-hong，et al(魏琴，李燕，段彩虹，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(1)：109.
[7] LIU Dian-mei，LI Shu-ting，ZHAO Shu-lin(刘典梅，李舒婷，赵书林). Chinese Journal of Analytical Chemistry(分析化学)，2004，32(2)：187.
[8] DU Bin，LI Yan，ZHANG Hui，et al(杜斌，李燕，张慧，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(7)：1110.
[9] LIU Hai-ping，WANG Xing-ming，DAI Ya-tang，et al(刘海萍，王兴明，戴亚堂，等). Chinese Journal of Inorganic Chemistry(无机化学学报)，2005，21(9)：1422.
[10] WANG Jing-yan，ZHU Sheng-geng，XU Chang-fa(王镜岩，朱圣庚，徐长法). Biochemistry (生物化学). Beijing：Higher Education Press(北京：高等教育出版社)，2002. 257.
[11] YE Bao-xian，ZHOU Xing-yao(冶保献，周性尧). Chemical Journal of Chinese Universities(高等学校化学学报)，1996，17(1)：33.
[12] HUANG Ying-ping，CAI Ru-xiu(黄应平，蔡汝秀). Chinese Journal of Analytical Chemistry(分析化学)，2001，29(4)：378.
[13] WANG Quan-lin，Lü Gong-xuan(王全林，吕功煊). Chinese Journal of Analytical Chemistry(分析化学)，2003，31(8)：945.
[14] WANG Quan-lin，LIU Zhi-hong，YUAN Hong，et al(王全林，刘志洪，原弘，等). Chinese Journal of Analytical Chemistry(分析化学)，2001，29(4)：421.
[15] HUANG Ying-ping，LIU Feng，CHEN Bai-ling，et al(黄应平，刘丰，陈百玲，等). Chinese Journal of Analysis Laboratory(分析试验室)，2003，22(1)：12.
[16] FENG Yu-ying，WU Xiao-hong，ZHOU Jia-hong，et al(冯玉英，吴晓红，周家宏，等). Applied Chemistry(应用化学)，2005，22(8)：895.
[17] YANG Pei-hui，ZHENG Zhi-wen，CAI Ji-ye(杨培慧，郑志雯，蔡继业). Chinese Journal of Luminescence(发光学报)，2004，25(3)：247.
[18] ZHENG Zhi-wen，YANG Pei-hui，FENG De-xiong，et al(郑志雯，杨培慧，冯德雄，等). Journal of Jinan University(Natural Science)(暨南大学学报·自然科学版)，2003，24(5)：106.
[19] White A，Handler P，Smith E L. Principles of Biochemistry(生物化学原理). Beijing：Science Press(北京：科学出版社)，1978. 110.
[20] KE Yi-kan，DONG Hui-ru(柯以侃，董慧茹). The Handbook of Analysis Chemistry(Spectral Analysis) (分析化学手册·第三分册光谱分析).The Second Edition(第2版). Beijing：Chemical Industrial Press(北京：化学工业出版社)，1998. 773.