荧光分析技术在作物响应镉胁迫研究中的应用

doi:10.3964/j.issn.1000-0593(2020)10-3118-05

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Abstract To reveal the application of fluorescence analysis technology on study of crop response to cadmium stress, the changes in cadmium concentration, chlorophyll fluorescence parameters, and fluorescence response curves of soybean seedlings under cadmium stress were analysed, and the relationship between chlorophyll fluorescence parameters and cadmium concentration in leaves was further assessed. The results showed that, with increased cadmium stress time, the concentration of cadmium in leaves increased gradually, reaching its maximum after 9 days of cadmium stress. After 1 day of cadmium stress, the PSⅡ reaction centre of soybean could dissipate excessive excitation energy by increasing non-photochemical quenching (NPQ) and decreasing actual efficiency of PSⅡ (Ф_PSⅡ) and apparent electron transport rate (ETR). Thus the physiological damages caused by energy over-excitation in PSⅡ reaction centre were avoided and the stability of the maximal efficiency of PSⅡ (F_v/F_m) was maintained, indicating that the dynamic photo-inhibition in soybean caused by cadmium stress then occurred. After 6 days of cadmium stress, NPQ continued to increase, but the photo-protection mechanism was insufficient to avoid the physiological damage caused by energy over-excitation. Thus, F_v/F_m of soybean seedlings showed a trend of decrease, indicating that soybean seedlings were then affected by chronic photo-inhibition. We next performed the correlation and regression analysis between chlorophyll fluorescence parameters and cadmium concentration. F_v/F_m was non-linearly correlated with cadmium concentration (R²=0.907, p<0.05), while Φ_PSⅡ (R²=0.959, p<0.01) and ETR (R²=0.945, p<0.01) were negatively correlated with cadmium concentration. In addition, NPQ and cadmium concentration was positively correlated (R²=0.959, p<0.01). These results indicated that F_v/F_m could maintain stability under certain cadmium stress, while Φ_PSⅡ, ETR and NPQ changed rapidly with increasing cadmium concentration. The changes in light response curves of chlorophyll fluorescence under cadmium stress were also analysed. It was found that the light response curves of Φ_PSⅡ and NPQ in stressed leaves showed similar trends to those observed in non-stressed leaves. However, under specific light intensity, Φ_PSⅡ in stressed leaves was lower than that of non-stressed leaves, while NPQ in stressed leaves was higher than that of non-stressed leaves, indicating that cadmium stress reduced the photochemistry activity of leaves, and caused more excitation energy dissipation as thermal. These studies confirmed that, fluorescence analysis technology could provide guidance for further study on the physiological mechanism of crop response to cadmium stress.

Key words：Crops; Cadmium stress; Fluorescence analysis; Fluorescence parameters; Fluorescence curves

Received: 2019-09-03 Accepted: 2020-01-21

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Q945.11

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http://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2020)10-3118-05 OR http://www.gpxygpfx.com/EN/Y2020/V40/I10/3118