|
|
|
|
|
|
Study on Interaction Between Phenylethanolamine A and CdTe Nanomaterials by Fluorescence Spectroscopy |
XIONG Yang1,2, XU Jun1,2, QIU Su-yan1,2, WEI Yi-hua1,2, ZHANG Jin-yan1,2* |
1. Institute for Quality & Safety and Standards of Agricultural Products Research, Jiangxi Acadeny of Agricultural Sciences, Nanchang 330200, China
2. MOA Laboratory of Quality Institute & Safety Risk Assessment for Livestock and Poultry Products(Nanchang), Nanchang 330200, China |
|
|
Abstract Phenylethanolamine A (PA) is a new adrenergic agonist and can improve feed utilization and the lean meat percentage of carcasses of livestock. But the drug residue would lead to a great threaten to human, such as nausea, dizziness,limbweakness, hand tremor and other symptoms of poisoning. Long-term consumption may lead to mutations in the body’s chromosomes, induced malignant tumorsetc. At present, the main detection method is liquid chromatography-tandem mass spectrometry (LC-MS-MS). The interaction between PA and CdTe nanomaterials is rarely reported. In this study, the water-soluble CdTe nanomaterials were prepared by microwave heating method,using mercaptopropionic acid as a stabilizer. The products have good fluorescence properties. The quantum yield of the CdTe nanomaterials was measured to be 0.523 4, and the half-peak width was about 45 nm. By using CdTe nanomaterials as fluorescent probe,based on the enhancement of the fluorescence intensity of CdTe nanomaterials by PA,a simple,rapid and sensitive method for the determination of PA was proposed and validated.Furthermore,the possible mechanism of interaction between CdTe nanomaterials and PA was discussed.Effects of experimental conditions were investigated. The optimal conditions were as follows: buffer: pH 7.4 KH2PO4-Na2HPO4 solution,concentration of PA:100 μg·L-1, reaction time: 15 min, reaction temperature: 20 ℃. Under the optimal conditions, a good linearity was obtained between fluorescence intensity and PA concentration in the range of 8~120 μg·L-1 with a correlation coefficient of 0.996.The obtained linear regression equation was: F/F0=0.001 9c+1.032 1 and the limit of detection was 3.5 μg·L-1.The proposed method was successfully applied in the determination of PA in swine urine samples, at the same time, the method is compared with the traditional liquid chromatography tandem mass spectrometry. The result indicated that the combination of CdTe nanomaterials and PA produced a nice labelling effect, and this method is fast, feasible and effective.
|
Received: 2019-04-24
Accepted: 2019-08-16
|
|
Corresponding Authors:
ZHANG Jin-yan
E-mail: zhangjinyana@126.com
|
|
[1] Elbert A H, Piet V Z, Aldo P, et al. Anal. Chem., 1998, 70: 1362.
[2] Howells L, Sauer M, Sayer R, et al. Anal. Chim. Acta, 1993, 275:275.
[3] Shao B, Jia X F, Zhang J, et al. Food Chemistry, 2009, 114: 1115.
[4] Wang P, Liu X, Su X, et al. Food Chem., 2015, 184: 72.
[5] Wang B B, Wang Q, Cai Z X, et al. LWT—Food Science and Technology, 2015, 61: 368.
[6] Announcement No.1519 of the Ministry of Agriculture of the People’s Republic of China(中华人民共和国农业部1519号公告) . 2010.
[7] SUN Wu-yong, ZHAO Bing-lin, ZHANG Shou-jie, et al(孙武勇, 赵冰琳, 张守杰, 等). Chinese Journal of Chromatography(色谱), 2012,30(10): 1008.
[8] XIANG Zi-lai(项自来). Development of Monoclonal Antibody to Phenylethanolamine A and Establishment of ELISA Method(苯乙醇胺A单克隆抗体的研制及ELISA检测方法的建立). Yangzhou:Yangzhou University(扬州:扬州大学), 2018.
[9] Ministry of Agriculture of the People’s Republic of China(中华人民共和国农业部). Determination of Phenylethanolamine A in Feed by High Performance Liquid Chromatography: NY/T3140—2017(饲料中苯乙醇胺A的测定 高效液相色谱法: NY/T3140—2017). Beijing: China Agricultural Press(北京: 中国农业出版社), 2018. 5.
[10] ZHANG Chun-yan, WANG Pei-long, SHI Lei, et al(张春艳, 王培龙, 石 雷, 等). Chinese Journal of Analytical Chemistry(分析化学), 2016, 44(12): 1859.
[11] Wu Le, Lin Zhengzhong, Zhong Huiping, et al. Food Chemistry, 2017, 229: 847.
[12] Raksawong Phannika, Chullasat Kochaporn, Nurerk Piyaluk, et al. Analytical and Bioanalytical Chemistry, 2017, 409: 4697.
[13] Xu Xixi, Dong Liu, Luo Lijun, et al. Sensors and Actuators B: Chemical, 2017, 251: 564.
[14] ZHANG Jin-yan,WEI Yi-hua,LIAO Qie-gen,et al(张金艳, 魏益华, 廖且根, 等). Quality and Safety of Agricultural Products(农产品质量与安全), 2015, (2): 51.
[15] Taylor D G, Demas J N, Taylor R P, et al. Biophysical Journal, 1978, 24(1): 77. |
[1] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[2] |
WU Lei1, LI Ling-yun2, PENG Yong-zhen1*. Rapid Determination of Trace Elements in Water by Total Reflection
X-Ray Fluorescence Spectrometry Using Direct Sampling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 990-996. |
[3] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, YUE Yuan-bo2, HU Xue-qiang2, CHEN Yu2, LI Xiao-jia1, 2*. The Detection of Mercury in Solutions After Thermal Desorption-
Enrichment by Energy Dispersive X-Ray Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1117-1121. |
[4] |
WEI Yi-hua1, HUANG Qing-qing2, ZHANG Jin-yan1*, QIU Su-yan1, 3, TU Tian-hua1, YUAN Lin-feng1, DAI Ting-can1, ZHANG Biao-jin1, LI Wei-hong1, YAN Han1. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3822-3827. |
[5] |
LIU Yu1, LI Zeng-wei2, DENG Zhi-peng1, ZHANG Qing-xian1*, ZOU Li-kou2*. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2817-2822. |
[6] |
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372. |
[7] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, HU Xue-qiang2, LIAO Xue-liang2, YUE Yuan-bo2, LI Xiao-jia1,2, CHEN Ji-wen3. The Rapid Detection of Trace Mercury in Soil With EDXRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 734-738. |
[8] |
LIU Chong-hua, OUYANG Yu, CHEN Guan-qian, PENG Cai-hong, SONG Wu-yuan. Rapid Determination of Chromium, Arsenic, Selenium, Cadmium, Antimony, Barium, Mercury and Lead in Toy Plastics by Energy Dispersive X-Ray Fluorescence Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 739-744. |
[9] |
ZHAO Ting1,2,3, CHI Hai-tao1,2,3*, LIU Yi-ren1,2,3, GAO Xia1,2,3, HUANG Zhao1,2,3, ZHANG Mei1,2,3, LI Qin-mei1,2,3. Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 750-754. |
[10] |
ZHAO Hong-kun1, 2, YU Tian3, XIAO Zhi-bo3, HAO Ya-bo4, LIU Ya-xuan1*. Homogeneity Test of Geochemical Certified Reference Materials by X-Ray Fluorescence Spectrometry With Pressed-Powder Pellets[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 755-762. |
[11] |
CHEN Hai-jie1, 2, MA Na1, 2, BO Wei1, 2, ZHANG Ling-huo1, 2, BAI Jin-feng1,2, SUN Bin-bin1, 2, ZHANG Qin1, 2, YU Zhao-shui1, 2*. Research on the Valence State Analysis Method of Selenium in Soil and Stream Sediment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 871-874. |
[12] |
CHEN Hai-jie1, 2, MA Na1, 2, BAI Jin-feng1, 2, CHEN Da-lei3, GU Xue1, 2, YU Zhao-shui1, 2, SUN Bin-bin1, 2, ZHANG Qin1, 2*. Study on Determination of Se in Geochemical Samples by External Supply H2-Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2896-2900. |
[13] |
ZHAO Yu-yan, ZHANG Ze-yu, TANG Xiao-dan*, ZANG Li-bin, LIU Xu-yang, LU Ji-long. Analysis of High Content Water-Soluble Salt Cation in Saline-Alkali Soil by X-Ray Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1467-1472. |
[14] |
LIN Hai-lan1, 2, ZHU Ri-long1*, YU Lei2, CHENG Yong-xia3, ZHU Rui-rui2, LIU Pei2, REN Zhan-hong3. Determination of Arsenic, Mercury, Selenium, Antimony and Bismuth in Soil and Sediments by Water Bath Digestion-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1528-1533. |
[15] |
TANG Xiao-yu1, LUO Yun-jing1*, LI Shu-guang2, LIN Tai-feng1, WANG Yan1. The Effects of Antioxidant, DPPH and Human Serum Albumin Ternary System Studied by Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3122-2128. |
|
|
|
|