| 光谱学与光谱分析 |
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| Fluorescence Spectroscopy Study of Humen Serum Albumin Quenched by Levofloxacin |
| CHEN Xiao-bo, KANG Dong-guo, LI Song，ZHAO Cheng-yi, CHEN Xiao-shan |
| Applied Optics Beijing Area Major Laboratory, Analytic and Testing Center, Beijing Normal University, Beijing 100875, China |
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Abstract The fluorescence spectroscopy of humen serum albumin(HSA) and the interaction of HSA and the Levofloxacin were studied. When the Levofloxacin was added into HSA solution gradually, an interesting new phenomenon emerged in emission spectrum peak. The intensity of 343.70 nm emission peak of HSA decreased obviously and moved towards long-wavelength, which is a typical quenching phenomenon. It was found that the excitation peak of HSA was positioned at 286.70 nm also. A new emission peak emerged at 503.96 nm, which resulted from the Levofloxacin. The excitation peaks of 503.96 nm fluorescence of Levofloxacin were positioned at 300.16 nm and 336.16 nm. When Levoflaxasin was added into HSA gradually, the 300.16 nm and 336.16 nm excitation peaks moved towards long wavelength. The dissociation constant of Levofloxacin from HSA is about Kd=3.65×10-5(mol·L-1). The combination constant of Levofloxacin is about KS=2.742×104(L·mol-1). The quenching process of Levofloxacin-HSA is not dynamic quenching, which resulted from the molecular diffusion and collision. It is caused by the static quenching process resulting from the chemical component between molecules. The energy transfer efficiency between Levofloxacin and HSA is E=0.372. According to these calculation results, the combination position between the binding site of Levofloxacin and the tryptophane of HSA is about R=1.933 nm.
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Received: 2004-05-18
Accepted: 2004-10-08
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Corresponding Authors:
CHEN Xiao-bo
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| Cite this article: |
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CHEN Xiao-bo,KANG Dong-guo,LI Song, et al. Fluorescence Spectroscopy Study of Humen Serum Albumin Quenched by Levofloxacin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1451-1455.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I09/1451 |
[1] HUANG Ben-li(黄本立). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2000, 20: 129.
[2] ZHOU Ya-qin, SONG Zeng-fu, XIAO Mei-ying, et al(周雅琴，宋增福，肖枚英，等). Acta Scientiarum Naturalium Unversitatis Pekinensis(北京大学学报(自然科学版)), 1996, 32(1): 53.
[3] MA Gui-bin, YANG Pin(马贵斌, 杨 频). Chinese Biochemical Journal(生物化学杂志), 1992, 8(5): 624.
[4] YANG Man-man, YANG Pin(杨曼曼, 杨 频). Chinese Science Bulletin(科学通报), 1994, 39(1): 31.
[5] YU Tian-zhi, TAO Zu-yi(俞天智, 陶祖贻). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(3): 453.
[6] WANG Jin-yan, ZHU Shen-geng, XU Chang-fa(王镜岩，朱圣庚, 徐长法). Biochemistry(生物化学). Beijing: Higher Education Press(北京: 高等教育出版社), 2003.
[7] Ware W R. J Phys. Chem., 1962, 66: 455.
[8] TAO Li-mei, HU Jie-han, ZHANG Yu-xin(陶丽梅, 胡皆汉, 张玉新). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1994, 14(3):45.
[9] He D C, Zeng C Q, Woods K, et al. Chromosoma, 1998, 107：189.
[10] Lakowicz J R, Weber G. Biochemistry, 1973, 12: 4161.
[11] ZHAO Chang-chun, ZHENG Wei-fa, LI Meng-qiu(赵长春，郑维发，李梦秋). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)：111.
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