|
|
|
|
|
|
Determination of Cuprous in Biological Samples by Liquid-Liquid Extraction-GFAAS |
ZHANG Yuan, WU Peng, LI Hui, LUO Hong-jun, LUO Wen-hong, LIN Zhe-xuan* |
Laboratory of Biological Analysis of Shantou University Medical College, Shantou 515041, China |
|
|
Abstract A method for the determination of cuprous [Cu(Ⅰ)] in biological samples by liquid-liquid extraction-inductively coupled plasma mass spectrometry (ICP-MS) was developed. Serum and cell homogenate, cell membrane components were deproteinized with trichloroacetic acid, then the supernatant was mixed with a glycine- sodium hydroxide (NaOH) buffer (pH 12.5) to obtain a mixture with pH of 9. Then 1 000 μL of 0.05% 2,2’-biquinoline dissolved in N-pentanol was added and vortexed for 1 min. After static layering, 500 μL of the organic layer was collected in a 2 mL Teflon digestive tube, and the organic solvent was evaporated in an oven at 95 ℃. After digestion with nitric acid and hydrogen peroxide, the sample solution was subjected to inductively coupled plasma mass spectrometry and the data of cuprous content were obtained. The detection limit of this method was 0.04 μg·L-1, with relative deviation being less than 5%. The recovery was 95% to 102%. Then the method was applied to determine Cu(Ⅰ) of serum from cervical cancer patients and several types of water. The results showed that serum Cu(Ⅰ) was higher in cervical cancer patients than that in normal controls. Cuprous ions could not be detected in tap water, Nongfu Spring water, and urine. While, Cuprous ions but not divalent copper ions could be detected in cytosol and cell membrane. This method could detect trace Cu(Ⅰ) ions in the presence of 10 times concentration of Cu(Ⅱ) ion without interference.
|
Received: 2018-12-18
Accepted: 2019-04-29
|
|
Corresponding Authors:
LIN Zhe-xuan
E-mail: g_zxlin@stu.edu.cn
|
|
[1] YU Yan, LI Hui-ling(于 燕,李惠玲). Journal of Environment and Health(环境与健康),2018, 35(3):272.
[2] Millhauser G. Annu. Rev. Phys. Chem., 2007, 58: 299.
[3] Squitti R, Barbati G, Rossi L. Neurology, 2006, 67: 76.
[4] Hussain S P, Raja K, Amstad P A. et al. Proc. Natl. Acad. Sci. USA, 2000, 97: 12770.
[5] Hellman N E, Gitlin J D. Annu. Rev. Nutr., 2003, 22: 439.
[6] Shi X, Stoj C, Romeo A, et al. J. Biol. Chem., 2003, 278: 50309.
[7] Lai Minchao, Wang Dian, Lin Zhexuan, et al. J. Stroke Cerebrovasc Dis., 2016, 25(1): 214.
[8] Fraústo da Silva J J R, Williams R J P. The Biological Chemistry of the Elements: The Inorganic Chemistry of Life. Oxford: Oxford University Press, USA, 2001. 600.
[9] Haiiiweii B,Gutteridge J M. Moi. Asp. Med.,1995,8:89.
[10] YANG Jing, LI Jian-qiang, LI Ren-yong(杨 竟, 李建强, 李仁勇). Chinese J. Anal. Chem.(分析化学), 2007, 35(9): 1393.
[11] Basheer C, Lee H K. Electrophoresis,2007,28(19): 3520.
[12] Zhang Yuan,Chen Yaowen,Lin Zhexuan,et al. Clinical Laboratory, 2014, 60(4): 543.
[13] CAO Xiao-xiao, LI Yan-qin(曹晓晓,李燕琴). Foreign Medcal Sciences·Section of Medgeography(国外医学医学·地理分册),2011, 32(4):277.
[14] Subhra Samanta, Nicolai Lehnert. Nature Chemistry, 2016, 18(7): 639.
[15] Brenner A J, Harris E D. Analytical Biochemistry, 1995, 226(1): 80.
[16] LI De-guang, DU Xiang-ge(李德广, 杜湘革). Journal of University Sciences(安徽农业科学),2009, 37(5):1897.
[17] ZHANG Yuan, LIN Zhe-xuan, LI Hui, et al(张 源,林哲绚, 李 慧, 等). Chinese J. Anal. Chem.(分析化学), 2004, 32(11): 1421.
[18] CAI Jun-jun, ZHU Xiang-kun, TANG Su-han(蔡俊军,朱祥坤,唐素寒). Journal of University Geology(高校地质学报),2006, 12(3):392.
[19] Holme D J, Peck H. Analytical Biochemistry. 3 rd. New York: Addison Wesley Longman Limited, 1998. 388.
[20] HU Wei-guo(胡卫国). Shijiazhuang Journal of Chemical Industry(石家庄化工), 1997, (2): 33. |
[1] |
LI Xiao-dian1, TANG Nian1, ZHANG Man-jun1, SUN Dong-wei1, HE Shu-kai2, WANG Xian-zhong2, 3, ZENG Xiao-zhe2*, WANG Xing-hui2, LIU Xi-ya2. Infrared Spectral Characteristics and Mixing Ratio Detection Method of a New Environmentally Friendly Insulating Gas C5-PFK[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3794-3801. |
[2] |
HUANG You-ju1, TIAN Yi-chao2, 3*, ZHANG Qiang2, TAO Jin2, ZHANG Ya-li2, YANG Yong-wei2, LIN Jun-liang2. Estimation of Aboveground Biomass of Mangroves in Maowei Sea of Beibu Gulf Based on ZY-1-02D Satellite Hyperspectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3906-3915. |
[3] |
GUO Na1, 2, WANG Xin-chen3*. Different Types of Deposits in Porphyry Metallogenic System Identified by 2 200 nm Al—OH Group Vibration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3492-3496. |
[4] |
CHEN Heng-jie, FANG Wang, ZHANG Jia-wei. Accurate Semi-Empirical Potential Energy Function, Ro-Vibrational Spectrum and the Effect of Temperature and Pressure for 12C16O[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3380-3388. |
[5] |
ZHANG Yu-hui1, 2, DING Yong-kang3, PEI Jing-cheng1, 2*, GU Yi-lu1, 2, YU Min-da1, 2. Chemical Constituents and Spectra Characterization of Monocrystal
Rhodonite From Brazil[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3504-3508. |
[6] |
WANG Yu1, 2, ZHANG Xian-ke1, 2, TAN Tu1, WANG Gui-shi1, LIU Kun1, SUN Wan-qi3*, QIU Zi-chen4, GAO Xiao-ming1, 2. Research on Moving Observation of Typical Greenhouse Gas Sources in Hefei by Using Off-Axis Integrated Cavity[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3293-3301. |
[7] |
ZHAO Ling-yi1, 2, YANG Xi3, WEI Yi4, YANG Rui-qin1, 2*, ZHAO Qian4, ZHANG Hong-wen4, CAI Wei-ping4. SERS Detection and Efficient Identification of Heroin and Its Metabolites Based on Au/SiO2 Composite Nanosphere Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3150-3157. |
[8] |
TAO Long-feng1, 2, LIU Chang-jiang2, LIU Shu-hong3, SHI Miao2, HAN Xiu-li1*. Preparation and Spectral Characteristics of Mn2+ Doped Nephrite Tailings Glass[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2710-2714. |
[9] |
HUANG Chao1, 2, ZHAO Yu-hong1, ZHANG Hong-ming2*, LÜ Bo2, 3, YIN Xiang-hui1, SHEN Yong-cai4, 5, FU Jia2, LI Jian-kang2, 6. Development and Test of On-Line Spectroscopic System Based on Thermostatic Control Using STM32 Single-Chip Microcomputer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2734-2739. |
[10] |
LIU Guo-peng1, YOU Jing-lin1*, WANG Jian1, GONG Xiao-ye1, ZHAO Yu-fan1, ZHANG Qing-li2, WAN Song-ming2. Application of Aerodynamic Levitator Laser Heating Technique: Microstructures of MgTi2O5 Crystal and Melt by in-situ Superhigh Temperature Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2507-2513. |
[11] |
LIU Zhao1, 2, LI Hua-peng1, CHEN Hui1, 2, ZHANG Shu-qing1*. Maize Yield Forecasting and Associated Optimum Lead Time Research Based on Temporal Remote Sensing Data and Different Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2627-2637. |
[12] |
ZHANG Zi-hao1, GUO Fei3, 4, WU Kun-ze1, YANG Xin-yu2, XU Zhen1*. Performance Evaluation of the Deep Forest 2021 (DF21) Model in
Retrieving Soil Cadmium Concentration Using Hyperspectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2638-2643. |
[13] |
YUAN Kai-xin, ZHUO Jin, ZHANG Qing-hua, LI Ya-guo*. Study on the Spectral and Laser Damage Resistance of CO2 Laser Modified Sol-Gel SiO2 Thin Films[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1752-1759. |
[14] |
WANG Shu-ying*, YOU De-chang, MA Wen-jia, YANG Ruo-fan, ZHANG Yang-zhi, YU Zi-lei, ZHAO Xiao-fang, SHEN Yi-fan. Experimental Collisional Energy Transfer Distributions for Collisions of CO2 With Highly Vibrationally Excited Na2[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1760-1764. |
[15] |
QIAN Duo, SU Wen-en, LIU Zhi-yuan, GAO Xiao-yu, YI Yu-xin, HU Cong-cong, LIU Bin, YANG Sheng-yuan*. Soy Protein Gold Nanocluster as an “Off-On” Fluorescent Probe for the Detection of Bacillus Anthracis Biomarkers DPA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1815-1820. |
|
|
|
|