光谱学与光谱分析 |
|
|
|
|
|
Sensitive Determination of Hemoglobin in Human Blood and Serum by Flow Injection Coupled with Chemiluminescence Detection |
CAO Jun-tao1, WANG Hui1, CHEN Yong-hong2, LIU Yan-ming1* |
1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China 2. Xinyang Central Hospital, Xinyang 464000, China |
|
|
Abstract A sensitive method to detect hemoglobin (Hb) by flow injection (FI) coupled with chemiluminescence (CL) detection was described, which based on Hb strong enhancing effect on weak luminol-hydrazine CL system in alkaline medium. Parameters affecting the CL detection conditions and FI-CL system were optimized. The effects of possible coexisting substances in human blood and serum of detection Hb were evaluated. Under the optimum conditions, the net CL intensity versus Hb concentration was linear in the range of 5.0×10-9~6.0×10-5 g·mL-1 with the detection limit of 5.8×10-10 g·mL-1 (9.0×10-12 mol·L-1). The relative standard deviations (RSDs) for 8 replicate determinations of 5.0×10-7 and 3.0×10-6 g·mL-1 Hb were 1.6% and 1.5%, respectively. In addition, the recoveries of Hb in human blood and serum were carried out and varied from 83.0% to 101.0%. The proposed method has been successfully applied for the determination of Hb in healthy human blood and serum. The possible mechanism of Hb enhancing the weak CL emission of luminol-hydrazine system in NaOH solution was discussed by fluorescence spectrophotometer and UV-Vis spectrophotometer.
|
Received: 2013-03-28
Accepted: 2013-06-25
|
|
Corresponding Authors:
LIU Yan-ming
E-mail: liuym9518@sina.com
|
|
[1] Shiao S Y P K, Ou C N. Hemoglobin, 2006, 30(4): 419. [2] Campbell T A, Ware R E, Mason M. Cytometry, 1999, 35(3): 242. [3] Parkes J, Ray R, Kerestan S. et al. Diabetes Technol. Ther., 1999, 1(4): 411. [4] Zhi Q, Xie C, Huang X Y, Ren J C. Anal. Chim. Acta, 2007, 583(2): 217. [5] Winichagoon P, Svasti S, Munkongdee T. et al. Transl. Res., 2008, 152(4): 178. [6] Tanaka T, Izawa K, Okochi M. et al. Anal. Chim. Acta, 2009, 638(2): 186. [7] Xu Y X, Hu C G, Hu S S. Anal. Chim. Acta, 2010, 663(1): 19-26. [8] Biroccio A, Urbani A, Massoud R. et al. Anal. Biochem., 2005, 336(2): 279. [9] Lu J S, Wei W, Yin L H, et al. Analyst, 2013, 138(5): 1483. [10] Liu Y M, Liu Z L, Shi Y M. Luminescence,2011, 26(1): 59. [11] Zhang S C, Ju H X. Anal. Chim. Acta, 2003, 475(1-2): 163. [12] Safavi A, Absalan G, Bamdad F. Anal. Chim. Acta, 2008, 610 (2): 243. |
[1] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
[2] |
GUO Jing-fang, LIU Li-li*, CHENG Wei-wei, XU Bao-cheng, ZHANG Xiao-dan, YU Ying. Effect of Interaction Between Catechin and Glycosylated Porcine
Hemoglobin on Its Structural and Functional Properties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3615-3621. |
[3] |
GUO Yuan1, 2, HUANG Yi-xiang3, 4, HUANG Chang-ping3, 4, SUN Xue-jian3, 5, LUAN Qing-xian1*, ZHANG Li-fu3, 5*. Analysis of Blood Oxygen Content in Gingival Tissue of Patients With
Periodontitis Based on Visible and Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2563-2567. |
[4] |
LIU Jin, FU Run-juan, HAN Tong-shuai*, LIU Rong, SUN Di. Spectral Analysis of Human Tissues Based on a Direct Effective
Attenuation Coefficient Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2746-2751. |
[5] |
SUN Dai-qing1, 2, XIE Li-rong1*, ZHOU Yan2, GUO Yu-tao1, CHE Shao-min2. Application of SG-MSC-MC-UVE-PLS Algorithm in Whole Blood Hemoglobin Concentration Detection Based on Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2754-2758. |
[6] |
WANG Jin-shuang1, FU Ying-ying1, FU Min-rui1, GAO Bin1*, ZHENG Da-wei1, CHANG Yu2. Study on Erythrocyte Sublethal Damage Under Different Shear Stress Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1811-1815. |
[7] |
ZHENG Pei-chao, ZHONG Chao, WANG Jin-mei*, LUO Yuan-jiang, LAI Chun-hong, WANG Xiao-fa, MAO Xue-feng. Evaluation of Flow Injection-Solution Cathode Glow Discharge With an Interference Filter Wheel for Spectral Discrimination[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 842-847. |
[8] |
ZHOU Ying1, BAI Yong-hui1, SONG Xu-dong1*, YAO Min3, WANG Jiao-fei1, SU Wei-guang1, YU Guang-suo1,2*. Application of Chemiluminescence in Spectral Diagnosis: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3358-3364. |
[9] |
LIU Song-yang1, LIU Guang-da1, LIU Zhuo-ya2, QIU Ji-qing3*, CAI Jing1, ZHU Zhan-peng3, ZHANG Cheng3, QI Yuan3, ZHANG Shang1. Research on Extended Kalman Filter in Extracting Cerebral Blood Flow Signals by Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(07): 2048-2053. |
[10] |
WU Sheng-yang1,2, HU Ren-zhi1,2*, XIE Pin-hua1,2, LI Zhi-yan1,2, LIU Xiao-yan3, LIN Chuan1,4, CHEN Hao1,2, WANG Feng-yang1,2, WANG Yi-hui1,5, JIN Hua-wei1,2. Real-Time Measurement of NOy (Total Reactive Nitrogen Oxide) by Cavity Ring Down Spectrometer (CRDS)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1661-1667. |
[11] |
QIU Jia-chu1,4, RUAN Ping2,4*, YONG Jun-guang3, FENG Bo-hua2, 4, HUANG Dai-zheng5, SHEN Hong-tao6. UV-Visible Absorption Spectra and FTIR of Hemoglobin of Healthy People and It Spectroscopic Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1425-1430. |
[12] |
LIU Yao, TAN Jian-guo*, GAO Zheng-wang. College of Aerospace Science and Engineering, National University of Defense Technology, Changsha 410073, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1018-1022. |
[13] |
YI Ding-rong1, ZHAO Yan-li1, KONG Ling-hua2*, WANG Wen-qi1, HUANG Cai-hong1. Miniature Snapshot Narrow Band Multi-Spectral Imaging Technology for Cervical Cancer Screening[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(01): 157-161. |
[14] |
WANG Zhong-yuan1, ZHANG Hong-kang2*, NI Zhi-xin1, LIU Jing-qin1, CHEN Xin1, LI Sheng-yong1, YANG Qi-jin2, ZHANG Can1. FI-KR-FAAS Twice Gas Isolation Elution Method for the Determination of Trace Lead in Marine Biological Samples[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3578-3582. |
[15] |
CAO Xuan1,2, CHU Dong-zhi1,2, LIU Yan1,2*, MA Ran1,2, ZHANG Shu-wei1,2, WU Ning1,2, SHI Qian1,2, MA Hai-kuan1,2. An On-Line Microfluidic Analysis System for Seawater Chemical Oxygen Demand Using Ozone Chemiluminescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3698-3702. |
|
|
|
|