Surface Enhanced Raman Spectroscopic Study on the Gold-Labeled Protein Self-Assembled Surface
CHAO Ke-fu1, 2, ZHANG You-lin1, KONG Xiang-gui1*, FENG Li-yun1, 2, LI Bing1, ZENG Qing-hui1, SONG Kai1, 2, SUN Ya-juan1
1. Key Laboratory of Excited State Processes, Changchun Institute of Optics，Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Abstract：The human IgG molecules were labeled with 13nm gold nanoparticles and the complex of the gold-labeled human IgG molecules was immobilized on a silicon surface modified by 3-aminopropyltriethoxysilane and glutaraldehyde. The method increases not only the tightness but also the surface coverage for immobilization of the complex and retains protein configuration well on the silicon surface. The self-assembled complex surface was observed by AFM. The complex aggregated on the silicon surface and the “island” monolayer of the complex was obtained. Meanwhile the SERS spectrum of the complex self-assembled “island” monolayer on silicon surface was presented. In the present paper, the gold labeled human IgG molecules were self-assembled on the silicon surface, SERS spectra of protein were obtained and as SERS active substrates were provided for the study of the protein molecules.
朝克夫1,2,张友林1,孔祥贵1*,冯力蕴1,2,李冰1,曾庆辉1,宋凯1,2,孙雅娟1 . Au标蛋白自组装表面增强拉曼光谱的研究[J]. 光谱学与光谱分析, 2007, 27(09): 1757-1760.
CHAO Ke-fu1, 2, ZHANG You-lin1, KONG Xiang-gui1*, FENG Li-yun1, 2, LI Bing1, ZENG Qing-hui1, SONG Kai1, 2, SUN Ya-juan1. Surface Enhanced Raman Spectroscopic Study on the Gold-Labeled Protein Self-Assembled Surface. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(09): 1757-1760.
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