Toxic Effect of Pb Coated on High Siliceous Mineral Fine Particles Towards Escherichia Coli
DONG Fa-qin1, 2*, QIN Yong-lian1, DAI Qun-wei1, 2, ZHAO Yu-lian1,2, LIU Ming-xue3, HOU Li-hua1, GUO Yu-ting3, XU Feng-qin1, LUO Zhao-pei1
1. Environment and Resource College, Southwest University of Science and Technology, Mianyang 621010, China
2. Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education, Mianyang 621010, China
3. Life Science and Engineering College, Southwest University of Science and Technology, Mianyang 621010, China
Abstract：In this study, quartz dusts and Pb(Ⅱ), the high siliceous mineral fine particles and main heavy metal pollutants in atmospheric particulate matter, were used as experimental materials to prepare Pb coated onto quartz dusts. Experiments were carried out to examine the oxidative stress response of Escherichia coli to PbCl2 and 1.6 g·L-1 Pb(Ⅱ) coated onto quartz dusts, and to explore the effect on E. coli surface groups and protein amide Ⅰ band secondary structure. The reduction of cells viability, glutathione depletion with a concomitant increase in reactive oxygen species and malondialdehyde levels demonstrated that Pb(Ⅱ) and Pb coated onto quartz dusts induce oxidative stress leading to cytotoxicity in E. coli. Moreover Pearson correlation analysis showed that there existed significant positive correlation (p<0.05) between bacteria toxicity and Pb(Ⅱ) exchange state content in the dusts. Exposures to Pb coated onto quartz dusts produced significant increases vs. both single quartz dusts and Pb(Ⅱ) in ROS/MDA (p<0.05). The influence of Pb(Ⅱ), Pb coated onto quartz dusts on E. coli surface groups focused on phosphate ester groups and surface polysaccharide molecules, amide Ⅰ band characteristic peak (1 600~1 700 cm-1) were fitted by the second derivative, deconvolution and line fitting technology, found that protein amide Ⅰ band beta sheets/alpha helices ratio decreased obviously after infected, the ratio decreased from 1.41 to 1.33, 1.27 and 1.22, respectively, after effects of Pb (Ⅱ) and Pb coated onto quartz dusts(Q-Pb-0/Q-Pb-3), suggesting that the structure of bacterial surface proteins were changed, which may affect the physiological activities of the bacteria. The results indicate that the oxidative damage effect may be an important toxicity mechanism of Pb(Ⅱ) coated onto quartz dusts, in addition, exposure to Pb(Ⅱ) coated onto quartz dusts can cause association oxidative stress on E. coli than either material alone.
Key words：Quartz dusts; Lead ion; Escherichia coli; Oxidative damage; FTIR; Protein secondary structure; Joint toxicity
董发勤，秦永莲，代群威，赵玉连，刘明学，侯丽华，郭玉婷，许凤琴，罗昭培. 载铅(Ⅱ)高硅质大气矿物细颗粒对大肠杆菌的毒性作用研究[J]. 光谱学与光谱分析, 2017, 37(07): 2014-2018.
DONG Fa-qin, QIN Yong-lian, DAI Qun-wei, ZHAO Yu-lian, LIU Ming-xue, HOU Li-hua, GUO Yu-ting, XU Feng-qin, LUO Zhao-pei. Toxic Effect of Pb Coated on High Siliceous Mineral Fine Particles Towards Escherichia Coli. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(07): 2014-2018.
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