Design of Rapid Detection System for Urotropine in Food Based on SERS
LI Wei1, FAN Xian-guang1,2*, WANG Xin1, TANG Ming1, QUE Jing1, HE Jian1, ZUO Yong3
1. School of Aerospace Engineering, Xiamen University, Xiamen 361005, China
2. University Key Laboratory of Sensing Technology of Fujian Province, Key Laboratory of Optoelectronic Sensor Technology of Xiamen, Xiamen 361005, China
3. Changcheng Institute of Metrology & Measurement, The 1st Metrology & Measurement Research Center of National Defense Science Industry of China, Beijing 100095, China
Abstract：Based on surface enhanced Raman spectroscopy (SERS) technology, the detection system for rapid fild determination of urotropine in food is proposed. The detecting system consists of multifunctional pretreating module, Roman optical module and embedded master control module. It completed the pretreatment of sample, the generation, collection and transmission of the Raman spectral data. The multifunctional pretreating module integrate centrifuge, ultrasound and volatile functions all together, reducing the equipments used in the pretreatment experiment. Besides, it is portable and convenient for operation. The Roman optical module we designed embodies the probe of Raman spectrometer and monochromator, and the numerical aperture of the aspherical lens we used in the optical system of the probe was 0.6. Besides, the monochromator was designed based on Asymmetrical crossed Czerny-Turner structure. The hardware part of the embedded master control module is made up of ARM and FPGA, and its peripheral circuits. The software part of the module is made up of real time operation system μC/OS-Ⅲ, graphics library emWin, file system FatFs and various types of equipment components. Thus, accomplished the collection, transmission and calculation of the Raman spectral data from CCD. The liquid colloidal gold nanoparticle (LCP-1) was used in the detection experiments of urotropine, while the characteristic peaks at 1 047 cm-1 was chosen as the identity information of urotropine. The result of detection experiments shows that the system has the ability to identify the urotropine accurately, the detection limit of urotropine standards was 0.01 mg·L-1, with detection limit of urotropine in yuba and rice-noodle was 0.5 mg·L-1. Besides, the testing time was less than 20 minutes,which meets the requirements for rapid field determination of urotropine in food．
Key words：SERS; Urotropine; Sample pretreatment; Detection system
李 韦，范贤光，王 昕，汤 明，阙 靖，何 坚，左 勇. 基于SERS的食品中乌洛托品快速检测系统设计[J]. 光谱学与光谱分析, 2017, 37(06): 1778-1783.
LI Wei, FAN Xian-guang, WANG Xin, TANG Ming, QUE Jing, HE Jian, ZUO Yong. Design of Rapid Detection System for Urotropine in Food Based on SERS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1778-1783.
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