Spectroscopic Studies on the Host Plant Selection Mechanism of Trabala vishnou gigantina Yang (Lepidoptera: Lasiocampidae)
WEN Dong-mei1, LU Peng-fei1, LUO You-qing1*, ZHOU Jin-chi2, GAO Yong-wei2
1. The Key Laboratory of Beijing for Control to Forest Pest, Beijing Forestry University, Beijing 100083, China 2. Analysis Test Center, Beijing Forestry University, Beijing 100083, China
Abstract：Trabala vishnou gigantina Yang (Lepidoptera: Lasiocampidae) is a polyphagous forestry pest whose periodic breaking out results in great economic damage including total crop failure to forestry and fruit production in China. In this study, in order to improve the understanding of the host plant selection mechanism of T. vishnou gigantina larvae, locust, caragana, willow, poplar, apricot and sea-buckthorn were used as potential host plants for the test. Two-way choice experiment method was used to study the T. vishnou gigantina Yang feeding preferences of the six kinds of plants. Moreover, the chemical component and physical structure of six plants were analyzed with Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). Among the six plants, T. vishnou gigantina larvae showed a strong preference for sea-buckthorn, followed by, apricot, willow, poplar, locust, and caragana. The FTIR analysis displayed that those six plants presented similar characteristic on absorption peak position, peak amount, and shape. The targets (1 154/1 733, 1 154/898) by FTIR showed that lipids and polysaccharide were major nutriments to affect the host plant selection of T. vishnou gigantina larvae. The XRD results showed that crystallinity index (CrI) also could affect the host plant selection of T. vishnou gigantina larvae. In this research, spectroscopy technology was firstly applied to the study of interactive relationship between insect and host, which would blaze a trail for intensive study of host plant selection mechanism of insect at molecular level.
[1] LIU Yong-qiao，WU Chun-sheng(刘永樵，武春生). Fauna Sinica Insecta Vol.47(中国动物志，昆虫纲，第47卷). Beijing：Science Press(北京：科学出版社)，2006. [2] LIU Yong-hua，ZHANG Yi-qiao，YAN Xiong-fei，et al(刘永华，张一巧，阎雄飞，等). Plant Protection(植物保护)，2013，39(2): 147. [3] Avery P B, Kumar V, Simmonds M S J, et al. Applied Entomology and Zoology, 2015, 50(1): 79. [4] Noriyuki Suzuki. Population Ecology，2015, 57(2): 293. [5] GAO Jun-ping，PANG Bao-ping，MENG Rui-xia, et al(高俊平，庞保平，孟瑞霞，等). Chinese Journal of Applied Ecology(应用生态学报)，2007，18(3): 701. [6] DENG Yan，CHANG Ming-shan，WU Yao-jun，et al(邓 艳，常明山，吴耀军，等). China Forestry Science and Technology(林业科技开发)，2013，27(1)：43. [7] Janakiraman N, Johnson M. Rom. J. Biopys，2015，25(2): 131 [8] QIN Xiao-ling，SHI Yan-cai，WEI Xiao, et al(覃小玲，史艳财，韦 霄，等). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，2012, 29(3)：1303. [9] Zhu L, O’Dwyer J P, Chang V S, et al. Bioresource Technology, 2008, 99(9): 3817. [10] Segal L，Creely J J，Martin A E. Textile Research Journal, 1959, 29(10): 786. [11] Sain M, Panthapulakkal S. Industrial Crops and Products, 2006, 23(1): 1. [12] Li R, Fei J, Cai Y, et al. Carbohydrate Polymers, 2009, 76(1): 94. [13] Li H, Yuan X, Zeng G, et al. Fuel Processing Technology, 2009, 90(5): 657. [14] Kumar R, Mago G, Balan V, et al. Bioresource Technology, 2009, 100(17): 3948. [15] FAN Wen-xiu，HOU Yu-xia，FENG Su-wei, et al(范文秀，侯玉霞，冯素伟，等). Journal of Henan Agricultural Sciences(河南农业科学)，2012，41(9)：31. [16] Xie G, Yang B, Xu Z, et al. PLos one, 2013, 8(1)：e50171.
