Separation of Chlorophyll Fluorescence from Scattering Light of Algal Water Based on the Polarization Technique
PANG Hui-fang1,2, WANG Lin2*, JIANG Ling-ling1, CHEN Yan-long2, WANG Bing-qiang3, XIONG De-qi1
1. College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China 2. National Marine Environmental Monitoring Center, Dalian 116023, China 3. Department of Information Engineering, Cangzhou Technical College, Cangzhou 061001, China
Abstract：Once chlorophyll molecules within the planktonic algae cells absorb light energy, they will release energy in the form of chlorophyll fluorescence emission. The elastic scattering light of particles in water is partially polarized, while chlorophyll fluorescence is unpolarized, so we can use the properties to separate the fluorescence signal from total scattering spectra for further retrieval the concentration of chlorophyll. But for coastal waters, the approach feasibility is still unclear. Based on this, we conducted the experiment in laboratory to analyze the influence of different concentrations of inorganic particles (IOP) and chlorophyll on the fluorescence extraction using polarization discrimination technique. The results indicate that, for algae water with different concentrations of IOP, the fluorescence peak will decrease while the concentration increase, but the retrieval result is still reliable when the concentration up to 300 mg·L-1. For algae water with different concentrations of chlorophyll, the concentration of chlorophyll more higher, the efficiency of extracted fluorescence using polarization method is better，for common water, this approach can still work. The study proves that the polarization method is also applicable for complex water; it is of great importance for further detecting the concentration of chlorophyll in coastal waters using remote sensing.
庞卉芳1,2，王 林2*，姜玲玲1，陈艳拢2，王炳强3，熊德琪1 . 基于偏振技术的藻类水体散射光中叶绿素荧光分离方法研究 [J]. 光谱学与光谱分析, 2017, 37(02): 486-490.
PANG Hui-fang1,2, WANG Lin2*, JIANG Ling-ling1, CHEN Yan-long2, WANG Bing-qiang3, XIONG De-qi1 . Separation of Chlorophyll Fluorescence from Scattering Light of Algal Water Based on the Polarization Technique. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(02): 486-490.
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