Laser-Induced Chlorophyll Fluorescence Spatial Variation Distribution and Model Establishment Based on Multi-Angle Spectroscopy and Polarization Spectroscopy
HAO Tian-yi, HAN Yang*, LIU Zi-ping, LI Zi-ying, ZHAO Yun-sheng, NIU Hao-fang, YAO Hai-yan
Key Laboratory of Geographical Processes and Ecological Security in Changbai Mountains, Ministry of Education, School of Geographical Sciences, Northeast Normal University, Changchun 130024, China
Abstract：According to the current research status, chlorophyll fluorescence is one of the important means of plant physiology research and plant remote sensing research. Previous studies on laser-induced fluorescence (LIF) have mostly used vertical directions, and less consideration has been given to the effects of multi-angle and fluorescence polarization. In this paper, Chlorophytum, Green radish, and Phnom Penh Tiger Piran were used as research objects, and multi-angle and polarization observation methods were used to analyze the laser-induced chlorophyll fluorescence spectra, and the models were established. At the same time, the spatial distribution of laser-induced chlorophyll fluorescence intensity and degree of polarization was plotted in polar coordinates. The results show that the shapes of fluorescence spectra of the three plants were different, and the changes in the Viewing Zenith Angles and Relative Azimuth Angles affect the fluorescence intensity and fluorescence parameters. The mathematical regression models of Viewing Zenith Angles and fluorescence parameter F685/F740 were established at Relative Azimuth Angles. According to the comparison of R2 and RMSE of different models, the Relative Azimuth Angle of 225° is the best angle to study the relationship between F685/F740 and Viewing Zenith Angles for these three plants at the same time. The regression coefficient is significant, which can accurately reflect that F685/F740 has a significant correlation with Viewing Zenith Angles. In addition, we also performed polarization observations on the laser-induced chlorophyll fluorescence of the three plants and calculated the corresponding fluorescence polarization in the 2π space. The studies have shown that the laser-induced chlorophyll fluorescence spectrum has significant polarization, and the Viewing Zenith Angles, Relative Azimuth Angles, and band information have different effects on laser-induced chlorophyll fluorescence polarization spectra. The mathematical regression models were established for the relationship between the fluorescence polarization at 685 and 740 nm for different plants. The regression coefficient is significant, which could accurately reflect the significant correlation between the fluorescence polarization at 685 and 740 nm.
郝天一，韩 阳，刘自平，李姿莹，赵云升，牛浩芳，姚海燕. 基于多角度光谱-偏振光谱的叶绿素荧光空间变化分布与模型建立[J]. 光谱学与光谱分析, 2020, 40(12): 3692-3698.
HAO Tian-yi, HAN Yang, LIU Zi-ping, LI Zi-ying, ZHAO Yun-sheng, NIU Hao-fang, YAO Hai-yan. Laser-Induced Chlorophyll Fluorescence Spatial Variation Distribution and Model Establishment Based on Multi-Angle Spectroscopy and Polarization Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3692-3698.
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