Study on the Interaction of PTB and Serum Albumin by Fluorescence Method
CHEN Yan-hua1, BU Feng-quan1, HU Xiu-li1, LIU Zhi-qiang2, LIU Zhong-ying1*, LIU Jing-ying1, ZHOU Qiu-li1
1. College of Pharmacology, Jilin University, Changchun 130021, China 2. New Drug R&D Division, Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Abstract：A molecular spectroscopic investigation of the interaction of phenacyl thiazolium bromide (PTB) and bovine serum albumin (BSA) or human serum albumin (HSA) is reported employing fluorescence quenching techniques. It is determined that the maximal excitation wavelength is 280 nm for BSA solution, and 290 nm for HAS solution. When PTB was added into these solutions gradually, the emission peaks were decreased obviously, which are typical quenching phenomena. The results obtained reveal that there is a medium-intensity binding affinity for PTB with HSA and BSA. At 15 ℃, the binding constants of PTB and BSA(HSA) are 3.66×103 and 3.83×103, and the numbers of binding sites are 1.02 and 1.06 respectively. At 37 ℃, the binding constants of PTB and BSA(HSA) are 3.58×103 and 3.35×103, and the numbers of binding sites are 0.95 and 0.87 respectively. According to the thermodynamic parameters, the main sort of the binding force between the drug and BSA or HSA was electrostatic force. Based on the Fster non-radiation energy transfer theory, it could be acquired that the distance between BSA or HSA and PTB is 7.5 or 7.9 nm. According to the crystal structure of serum albumin, it can be speculated that subdomain ⅡA was the binding sites for the interaction of PTB and serum albumin, which is the region near Try214.
陈艳华1,卜凤泉1,胡秀丽1,刘志强2,刘忠英1*,刘景英1,周秋丽1 . 荧光法研究PTB与白蛋白的结合[J]. 光谱学与光谱分析, 2007, 27(10): 2079-2082.
CHEN Yan-hua1, BU Feng-quan1, HU Xiu-li1, LIU Zhi-qiang2, LIU Zhong-ying1*, LIU Jing-ying1, ZHOU Qiu-li1 . Study on the Interaction of PTB and Serum Albumin by Fluorescence Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(10): 2079-2082.
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