Fluorescence Spectroscopy Study on Photobleaching Properties of Photosensitizers in Photodynamic Therapy
WANG Lei1, GU Ying1*, LI Xiao-song1, LIU Fan-guang1, YU Chang-qing2
1. Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China 2. Department of Optical Engineering, Beijing Institute of Technology, Beijing 100081, China
Abstract：The photobleaching and formation of photoproduct of hematoporphyrin monomethyl ether (HMME) was investigated in solution by fluorescence spectroscopy. Irradiation was performed by 532nm double-frequency Nd∶YAG laser at a fluence rate of 100 mW·cm-2, meanwhile the collection of fluorescence spectra was implemented an by optical multi-channel analyzer (OMA). Utilizing the basis spectra constructed and least-square fitting, the intensity of HMME fluorescence (613 nm), photoproduct fluorescence (639 nm) and autofluorescence can be resolved respectively from the single measured spectrum. The correlation between fluorescence intensity and the concentration of HMME is consistent with linear function at concentrations not exceeding 10 μg·mL-1. Monitoring the fluorescence spectroscopy during irradiation, three kinds of information were obtained, including photobleaching of HMME, formation and bleaching of photoproduct, as well as variation to fluorescence intensity due to the change of sample optical properties. Product generated by bleaching of photoproduct causes significant in sample optical properties. The implementation of fluorescence spectroscopy measurement and spectral analysis method presented here can be utilized effectively for both ex vivo investigation of photobleaching characteristics of photosensitizer and in vivo monitoring for dosimetry in photodynamic therapy.
王雷1,顾瑛1*,李晓松1,刘凡光1,于常青2 . 荧光光谱用于光动力疗法中光敏剂光漂白特性研究[J]. 光谱学与光谱分析, 2007, 27(10): 2073-2078.
WANG Lei1, GU Ying1*, LI Xiao-song1, LIU Fan-guang1, YU Chang-qing2. Fluorescence Spectroscopy Study on Photobleaching Properties of Photosensitizers in Photodynamic Therapy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(10): 2073-2078.
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