Determination of 20 Trace Elements in the Blood Collection Tubes with ICP-MS
XIONG Chan1, 5, JIANG Xue-hui1, TIAN Ya-ping2, MA Qing-wei3, LIU Li-peng4, GUO Guang-hong2*
1. Department of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin 300072, China 2. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, China 3. Bioyong Technology Inc, Beijing 102206, China 4. Focused Photonics Inc, Hangzhou 310052, China 5. Institution of Chemistry, Karl-Franzens University Graz, Graz A-8010, Austria
Abstract：To investigate the contamination of blood collection tubes, 20 trace elements (Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Sn, Sb, Ba, W, Hg, Tl, Pb) in 13 different types of blood collection tube were studied with ICP-MS method. The lixivium of H2O and 10% HNO3 were measured with ICP-MS, and then the contamination coming from the blood collection tube is specified. According to the concentration range of human blood, plasma and serum from recently published literature, this report presents a detailed analysis of capable trace elements for each blood collection tube. The results showed that, tube No.1 is capable to analyze 18 trace elements in the human serum; tube No.6 is capable to analyze 15 trace elements in the human plasma; tube No. 13 is capable to analyze 17 trace elements in the human blood. But we still should be aware that, the elements Sb and W in tube No.1, the elements V, Cr, Ni, and Sb in tube No.6, and the elements Al, Sb and W in tube No.13, are in the same magnitude of the normal trace element concentration range in the human serum, plasma and blood. They might affect the testing results. The serum collected from the same volunteer by tube No.1 and tube No.3 were compared here, the results show that, almost each trace element concentration of human serum from tube No.1 is lower than from tube No.3, especially for elements Al, V, Cr, Mn, As, Sn, and Sb. The results indicate that the blood collection tubes show great impact on determination of trace element.
Key words：ICP-MS;Blood collection tube;Trace element
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