Abstract：In the present study, a synchronous spectrofluorimetric method has been applied to the simultaneous determination of vitamin B1, B2 and B6. However, their spectra are overlapped so it is difficult to determine them individually. The parallel factor analysis (PARAFAC) was applied to the resolution of the overlapped spectra of mixtures of vitamin B1, B2 and B6. The Δλ range used was from 20 to 120 nm, and the excitation wavelength range was selected from 200 to 500 nm. From the measured fluorescence data a four-factor PARAFAC model was established, and was applied to the determination of a set of synthetic validation samples and some commercial samples with satisfactory results. This work shows that PARAFAC method is a powerful chemometric tool to the determination of mixtures of vitamin B1, B2 and B6.
Key words：PARAFAC;Synchronous fluorescence;Vitamin B
倪永年，蔡英俊. 平行因子-同步荧光法测定食品中维生素B1，B2和B6[J]. 光谱学与光谱分析, 2005, 25(10): 1641-1644.
NI Yong-nian, CAI Ying-jun. Simultaneous Synchronous Spectrofluorimetric Determination of Vitamin B1, B2 and B6 by PARAFAC . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1641-1644.
