The Research on Measurement System and Method of Tissue Optical Parameters with Wide Spectra Based on Double-Integrating-Spheres
HAN Lei1, LI Chen-xi1, 2*, SUN Cheng-tao1, JIANG Jing-ying1, ZHAO Hui-juan1,2, XU Ke-xin1,2
1. College of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China 2. State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072, China
Abstract：The measurement of tissue optical parameters is the focusing research content of Biomedical Photonics. The optical properties of human tissue are closely related to the physiological and pathological state. In recent years, the tissue imaging diagnosis and non-invasive detection of componentsbecome the hot research topics, applying the tissue optical properties especially the absorption and scattering properties. These provide the basis for the study of optical imaging and the spectrum detection of body composition etc. The Double-Integrating-Spheres (DIS) method can measure the absorption coefficient, scattering coefficient and so on in vitro tissuesimultaneously. It has the advantages of accurate, rapid, large applicable scope. The method applya standard method for measuring the optical parameters. This paper build the wide spectrum measurement system of optical parameters based on DIS and super continuum lasers. Then we analyze the transfer function, error sources and the best measuring conditions of the system. Finally we establish the correction forward model based on BP-MCML and the inverse algorithm of the optical parameters based on L-M algorithm. The optical parameters of intralipid solution in the wavelength range of 1 100～1 400 nm are measured. The experiment results show that the improved inverse algorithm is accurate. The multiple measurements standard deviation is within 3%. Compared the results of scattering coefficient and absorption coefficient at different wavelengths to the results of other research groups, the deviation is less than 3.4%.
韩 蕾1，李晨曦1,2*，孙承涛1，蒋景英1，赵会娟1,2，徐可欣1,2 . 基于双积分球的宽光谱组织光学参数测量系统与方法研究 [J]. 光谱学与光谱分析, 2016, 36(02): 561-566.
HAN Lei1, LI Chen-xi1, 2*, SUN Cheng-tao1, JIANG Jing-ying1, ZHAO Hui-juan1,2, XU Ke-xin1,2 . The Research on Measurement System and Method of Tissue Optical Parameters with Wide Spectra Based on Double-Integrating-Spheres. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(02): 561-566.
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