摘要： Three simple, specific, accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate (AM) and atenolol (AT) in tablets. The first method is dual wavelength spectrophotometry (DW). The second method is ratio subtraction (RS) which depends on subtraction of the plateau values from the ratio spectrum, coupled to first derivative of ratio spectra (1DD). The third method applies bivariate calibration method using 210 and 225 nm as an optimum pair of wavelength for amlodipine and atenolol. The calibration curves are linear over the concentration range of 4～40 μg·mL-1 for both drugs. The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form. The two methods are validated as per ICH guidelines and can be applied for routine quality control testing.
Abstract：Three simple, specific, accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate (AM) and atenolol (AT) in tablets. The first method is dual wavelength spectrophotometry (DW). The second method is ratio subtraction (RS) which depends on subtraction of the plateau values from the ratio spectrum, coupled to first derivative of ratio spectra (1DD). The third method applies bivariate calibration method using 210 and 225 nm as an optimum pair of wavelength for amlodipine and atenolol. The calibration curves are linear over the concentration range of 4～40 μg·mL-1 for both drugs. The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form. The two methods are validated as per ICH guidelines and can be applied for routine quality control testing.
通讯作者:
Nesrine T. Lamie
E-mail: nesrinelamie@hotmail.com;nesrine.rezkalla@pharma.cu.edu.eg
引用本文:
Nesrine T. Lamie. Spectrophotometric Methods for Simultaneous Determination of Amlodipine Besylate and Atenolol in Their Tablet Dosage Form[J]. 光谱学与光谱分析, 2015, 35(12): 3538-3543.
Nesrine T. Lamie. Spectrophotometric Methods for Simultaneous Determination of Amlodipine Besylate and Atenolol in Their Tablet Dosage Form. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(12): 3538-3543.
[1] The Merck Index, Merck Research Laboratories; White House Station, 14th ed., 2006. [2] McEvoy G K. American Hospital Formulary Service American Society of Health-System Pharmacists Inc., Bethesda, 2001. [3] Sweetman S C. Martindale the Complete Drug Reference, 34th ed., Pharmaceutical Press, London, 2005. 862. [4] The British Pharmacopoeia. Her Majesty’s Stationery Office, London, 2010. [5] Rockville M D. The United State Pharmacopoeia, USP28-NF23 ed., United States Pharmacopoeial Convention Inc., 2009. [6] Audhari B G. AB Patel. Int. J Chem Tech Res.，2010，633. [7] European Pharmacopoeia, 2nd Ed., Council of Europe, 1995. [8] Barman R K, Islam M A, Ahmed M, et al. J. Pharm. Sci., 2007，20(4)：274. [9] Argekar A P，Powar S G. J. Pharm. Biomed. Anal., 2000，21(6)：1137. [10] Rathee P, Rathee S, Thakur S, et al. Int. J. of Chem. Tech. Research, 2010, 2(1): 62. [11] El-Bardicy M G, Lotfy H M, El-Sayed M A, et al. J AOAC Int.，2008，91：299. [12] Lopez-de-Alba P, López-Martínez L, Wrobel-Kaczmarczyk K, et al. Anal. Lett.，1996，29：487. [13] Massar D L, Vandeginste B G M, Deming S N, et al. Chemometrics: A Textbook, Elsevier, Amsterdam，1988. 124. [14] Spiegel M R，Stephens L J. Schaum Outline of Theory and Problems of Statistics, Schaum Outline Series, New York, 1999.
