Study on the Characters of Phytoplankton Chlorophyll Fluorescence Excitation Spectra Based on Fourth-Derivative
LU Lu1,SU Rong-guo1,2,WANG Xiu-lin1,2*,ZHU Chen-jian1,2
1. Marine Polluted Eco Chemistry Lab, Ocean University of China, Qingdao 266003, China 2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266003, China
Abstract：Chlorophyll fluorescence excitation spectra of six phytoplankton species, belonging to Bacillariophyta and Dinophyta, were dealt by fourth-derivative analysis with the Matlab program. The results show that between 350 nm and 550 nm six fluorescence peaks were found in the fourth-derivative spectra, which are representatives of non-pigments, chlorophylls and carotenoides respectively. The method makes Bacillariophyta and Dinophyta more distinguishable when the fourth-derivative spectra are compared with the chlorophyll fluorescence excitation spectra. It can be used not only to discriminate the two groups of algaes, but also to reduce the effect of noise. The fluorescence peaks in the fourth-derivative spectra are proved to be stable.
卢璐1,苏荣国1,2,王修林1,2*,祝陈坚1,2. 基于四阶导数的浮游植物叶绿素荧光激发光谱特征研究[J]. 光谱学与光谱分析, 2007, 27(11): 2307-2312.
LU Lu1,SU Rong-guo1,2,WANG Xiu-lin1,2*,ZHU Chen-jian1,2. Study on the Characters of Phytoplankton Chlorophyll Fluorescence Excitation Spectra Based on Fourth-Derivative. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2307-2312.
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