1. Institute of Biochemical Analysis, Xinzhou Teachers University, Xinzhou 034000, China 2. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China
Abstract:The interaction of two kinds of sulfated chitosans with DNA was investigated using fluorescence spectra,absorption spectra, alkaline denaturation curves, ionic strength, fluorescence quenching etc. The results showed that there were two modes of the interaction between sulfated chitosans and DNA/ ethidium bromide(EB): intercalative and electrostatic binding. Hyperchromic and bathochromic effects in the absorption spectra were observed when sulfated chitosans with a low degree of substitution (CT-L) or a high degree of substitution (CT-L) were bound to herring sperm DNA. The stability of DNA/EB was increased with the increase in alkaline denaturation pH in the presence of CT-H and CT-L. Meanwhile, a weak interaction of cationic Mg2+ and phosphate groups of DNA by electrostatic force binding was observed. The fluorescence was efficiently quenched in the presence of a micro-amounts of sulfated chitosans, which confirmed that there was a strong competitive binding interaction among EB, CT-H or CT-L and DNA. All of these demonstrated that different sulfated chitosans can be used as a type of hopeful target-pointed molecule of gene therapy.
张海容1,2,郭祀远2,李琳2,蔡妙颜2,郑必胜2 . 光谱法研究硫酸酯化壳聚糖与DNA的作用机理[J]. 光谱学与光谱分析, 2007, 27(11): 2294-2298.
ZHANG Hai-rong1,2,GUO Si-yuan2,LI Lin2,CAI Miao-yan2,ZHENG Bi-sheng2 . Study on the Binding Mode of Sulfated Chitosans with Nucleic Acids Using Spectral Analysis. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2294-2298.
[1] JIN Lan, YANG Pin, LI Qing-shan(靳 兰,杨 频,李青山). Chemical Journal of Chinese Universities(高等学校化学学报),1996, 17(9): 1345. [2] LI Lai-sheng, HUANG Wei-dong, WANG Li-ping, et al(李来生, 黄伟东, 王丽苹, 等). Acta Chimic Sinica(化学学报), 2002, 60(1): 98. [3] LI Wen-you, ZHU Shou-tian, HE Xi-wen, et al(李文友, 朱守田, 何锡文, 等). Acta Chimic Sinica(化学学报), 2002, 60(1): 105. [4] HE Ji-xiang, JIANG Chong-qiu, WANG Jin-shan(贺吉香, 江崇球, 王金山). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(1): 103. [5] Chirkov S N. Applied Biochemistry and Microbiology, 2002, 38(1): 1. [6] Xue C H, Fang Y, Lin H, et al. Journal of Applied Phycology, 2001, 13: 67. [7] Keisuke K. Polymer Degradation and Stability, 1998, 59: 117. [8] Krishna A Gopala, Kumar D Vijay, Khan B M, et al. Biochimica et Biophysica Acta, 1998, 1381: 104. [9] ZHANG Wei-jie(张惟杰). Biochemical Research Technology of Sugar Compound, 2nd ed.(糖复合物生化研究技术, 第2版). Hangzhou: Zhejiang University Press(杭州: 浙江大学出版社),1999. 291. [10] Gamzazade A, Sklyar A, Nasibov S, et al. Carbohydrate Polymers, 1997, 34: 113. [11] WANG Ai-qin, ZHAO Pei-qing, GAO Xiao-jun, et al(王爱勤, 赵培庆, 高小军, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(6): 817. [12] HU Min, ZHANG Zhen-xi, SHEN Guo-li, et al(胡 敏, 张镇西, 沈国励, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(9): 1668. [13] SHEN Jing-shan, SUN Dan-dan, FU Lian-chun, et al(沈景山,孙丹丹,付连春,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(2):232. [14] JIA Mu-xin, QU Wen-wen. CHEN Guang-ju, et al(贾木欣,曲文文,陈光巨,等). Chemical Journal of Chinese Universities(高等学校化学学报),2004,25(8):1481. [15] HUANG Su-lan, HAO Lan, TAN Hong-wei, et al(黄素兰,郝 兰,谭宏伟,等). Chemical Journal of Chinese Universities(高等学校化学学报),2006, 27(3): 531. [16] ZHANG Hai-rong, WU Xiao-yan(张海容,武晓燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(2):346. [17] ZHANG Hai-rong, GUO Si-yuan, LI Lin, et al(张海容,郭祀远,李 琳,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(4):711. [18] WANG Ping-hong, ZHANG Qi, YUAN Wen-bing, et al(王平红,张 歧,袁文兵,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(7):1298. [19] ZHANG Xiao-ming, LI Guo-xue, LI Dong-hao, et al(张晓明,李国学,李东昊,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(3):517.