1. College of Chemistry and Material Science，Experimental Center, Fujian Normal University，Fuzhou 350007，China 2. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education，Fujian Normal University，Fuzhou 350007，China
Abstract：The quenching reaction of hemoporphyrin derivative with hemoglobin (Hb) was studied by using fluorescence spectra and absorption spectra.It was shown that HpD has a powerful ability to quench the Hb fluorescence via a nonradiative energy transfer mechanism. The formation constants of them were analyzed at different temperature according to Stern-Volmer equation and double-reciprocal equation，which are bigger at high temperature than at low temperature. The critical binding site was calculated (R0＝3.22 nm) by Fster energy transfer mechanism, and the thermodynamic parameters were obtained.
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