Effect of Temperature on the Aggregation Behavior of Collagen Solution by Two-Dimensional Synchronous Fluorescence Correlation Spectroscopy
WU Wan-ye1, WU Kun2, LI Guo-ying1，2*
1. The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China 2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China
Abstract：The synchronous fluorescence spectroscopy and two dimensional correlation analysis method were applied to study the aggregation behavior of acid-soluble collagen solutions (0.2, 0.4 and 1.6 mg·mL-1) during the heating process of 10～70 ℃. It was found that the fluorescence excited at 292 and 282 nm (Δλ=9 nm) belongs to the tyrosine (Tyr) residues which participate in forming hydrogen bonds or not, respectively. The two dimensional correlation analysis with the temperature varying showed that with the temperature increased (10～30 ℃) hydrogen bonds among collagen molecular with Tyr residues formed in the 0.2 mg·mL-1 collagen solution, while the higher aggregations of collagen molecular and hydrophobic micro-domains appeared in the 0.4 and 1.6 mg·mL-1 collagen solutions. With approaching the denatured temperature of collagen (36～38 ℃), the hydrophobic micro-domain and aggregates seemed to be broken in the 0.4 and 1.6 mg·mL-1 collagen solutions, however the hydrogen bonds in the 0.2 mg·mL-1 were stable. Above the denaturation temperature of collagen, the triple-helix structure of collagen molecular in solution of each concentration tended to be loose. In the heating process of 45～70 ℃, this trend was more obvious.
Key words：Type I collagen;Temperature;Synchronous fluorescence spectroscopy;Two-dimensional correlation spectroscopy;Aggregation behavior
吴万烨1，武 昆2，李国英1，2* . 利用二维同步荧光相关光谱研究温度对胶原溶液中分子聚集行为的影响 [J]. 光谱学与光谱分析, 2015, 35(02): 409-414.
WU Wan-ye1, WU Kun2, LI Guo-ying1，2* . Effect of Temperature on the Aggregation Behavior of Collagen Solution by Two-Dimensional Synchronous Fluorescence Correlation Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(02): 409-414.
[1] LI Guo-ying, LIU Wen-tao(李国英，刘文涛). Collagen Chemistry(胶原化学). Beijing: China Light Industry Press(北京：中国轻工业出版社), 2013. 1. [2] Schmitt F O, Gross J, Highberger J H, Experimental Cell Research, 1955, 3: 326. [3] Wu Kun, Liu Wentao, Li Guoying. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2013, 102: 192. [4] Fan Daidi, Xing Jianyuan, Xue Wentao, et al. Chinese Journal of Chemistry, 2011, 29(9): 1811. [5] Julian M Menter. Photochem. Photobiol. Sci., 2006, 5: 403. [6] Lloyd J B F. Journal of the Forensic Science Society, 1971, 11(2): 83. [7] Digambara Patra, Mishra A K. Trends in Analytical Chemistry, 2002, 21(12): 787. [8] Liu Yongliang, Yukihiro Ozaki, Noda I. The American Journal of Physics，1996, 100(18): 7326. [9] Noda I, Dowrey A E, Marcott C, et al. Applied Spectroscopy, 2000, 54(7): 236. [10] Noda I. Applied Spectroscopy, 1993, 47(9): 1329. [11] PENG Xian-neng, CHEN Xin, WU Pei-yi, et al(彭显能，陈 新，武培怡，等). Acta Chimaca Sinaca(化学学报), 2001, 62(21): 2127. [12] Li Yang, Li Yanwen, Du Zongliang, et al. Thermochimica Acta, 2008, 469(1-2): 71. [13] Shae B Padrick, Andrew D Miranker. Journal of Molecular Biology, 2001, 308(4): 783. [14] Mu Changdao, Li Defu, Lin Wei, et al. Biopolymer, 2007, 86(4): 282. [15] Liu Wentao, Li Guoying. Polymer Degradation and Stability, 2010, 95(12): 2233.
