Beijing University of Chinese Medicine, Key Laboratory of TCM-Information Engineering of State Administration of TCM, Beijing Key Laboratory for Basic and Development Research on Chinese Medicine, Beijing 100102, China
Abstract：The optimization method was established to investigate the effect of near infrared chemical imaging (NIR-CI) detection parameters on hyperspectral data quality. In order to optimize the detection parameters, chlorpheniramine maleate (CPM) tablets were chosen as examples and the L9(34) orthogonal-test design was adopted to research the effects of spectral resolution, spatial resolution, scan times and scan height. Binary image coupled with statistical measurement was proposed to quantitatively analyze hyperspectral data and determine the content of CPM on the tablet surface. High-performance liquid chromatography (HPLC) was used as reference method for accurate CPM determination. The absolute value of the difference between CPM contents obtained from NIR-CI and HPLC was chosen as index. The result demonstrated that the optimum parameters for acquiring hyperspectral data were: 25 μm×25 μm (spatial resolution), 5340 (scan height, the value of Z, precise focus), 16 cm-1(spectral resolution) and 16 (scan times). The influence of scan height on hyperspectral data was firstly investigated. The optimized parameters could be applied to CPM tablets and other drugs for NIR-CI data acquisition and methodology establishment.
Key words：Near infrared chemical imaging;Optimization of detection parameters;Chlorpheniramine maleate tablet;Scan height
[1] Katherine A B. Process Analytical Technology. 2nd ed. John Wiley & Sons, 2010. 187. [2] Lewis E N, Schoppelrei J, Lee E. Spectroscopy, 2004, 19(4): 26. [3] Ozaki Y. Analytical Sciences, 2012, 28(6): 545. [4] Gendrin C, Roggo Y, Collet C. Journal of Pharmaceutical and Biomedical Analysis, 2008, 48(3): 533. [5] Gowen A A, O’Donnell C P, Cullen P J, et al. European Journal of Pharmaceutics and Biopharmaceutics, 2008, 69(1): 10. [6] Rozo J, Zarow A, Zhou B, et al. Journal of Pharmaceutical Sciences, 2011, 100(11): 4888. [7] Rosas J G, Armenta. S, Cruz. J, et al. Analytica Chimica Acta, 2013, 787: 173. [8] Franch-Lage F, Amigo J M, Skibsted E, et al. International Journal of Pharmaceutics, 2011, 411: 27. [9] Ma H, Anderson C A. Journal of Near Infrared Spectroscopy, 2009, 15(3): 137. [10] SaSic S. Analytica Chimica Acta, 2008, 611(1): 73. [11] Maurer L, Leuenberger H. International Journal of Pharmaceutics, 2009, 370(1): 8. [12] Kirkegaard J, Secher C, Mygind N. Allergy, 1982, 37(3): 203. [13] Vidal M, Amigo J M. Chemometrics and Intelligent Laboratory Systems, 2012, 117: 138.
