Abstract：Arundo donax linn, as a perennial energy crop, has promising application prospect. In the present study, Fourier transform Raman (FT Raman) spectroscopy was applied to determine the structural information of materials, milled wood lignin (MWL), and alkaline lignins (AL, under different treated time) from A. donax stem nondestructively. The results indicated that, extractable compounds in A. donax had negative contribution to the Raman spectra without rising new Raman peaks. FT Raman spectrum of MWL indicated that MWL from A. donax was HGS type lignins. Compared with the spectra of MWL from wood materials, the peak at 1 173 cm-1 was much higher in intensity for the MWL from A. donax stem, which may be assigned to hydroxycinnamic acid by analyzing the standard. With respect to FT Raman spectra of ALs, the relatively highest intensity of 1 173 cm-1 was found in alkaline lignin (AL2), which was treated for 40 min by alkaline. Moreover, the peak of coniferaldehyde/sinapaldehyde (1 630 cm-1) was lowest in intensity while the band attributed to coniferyl alcohol/sinapyl alcohol (1 660 cm-1) was almost disappeared in AL2. It could be inferred that AL2 demonstrated a highest content of phenolic acid, which may improve its potential application, such as for antioxidant activity. Furthermore, the results obtained by FT Raman spectra were verified by two dimensional heteronuclear singlequantum coherence nuclear magnetic resonance analyses. Above all, FT Raman spectroscopy provided alternative safe, rapid, accurate, and nondestructive technology for lignin structure determination.
游婷婷，马建锋，郭思勤，许 凤* . 傅里叶变换拉曼光谱对芦竹碱木质素的研究 [J]. 光谱学与光谱分析, 2014, 34(08): 2112-2116.
YOU Ting-ting, MA Jian-feng, GUO Si-qin, XU Feng* . Study of Alkaline Lignin from Arundo donax Linn Based on FT Raman Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(08): 2112-2116.
[1] Argyropoulos D S, Menachem S B. Biopolymers from Renewable Resources. Berlin: Springer, 1998. 292. [2] Buranov A U, Mazza G. Industrial Crops and Products, 2008, 28(3): 237. [3] ZHOU Yan-yan(周艳艳). China Pulp & Paper Industry(中华纸业), 2010, 31(14)： 82. [4] You T T, Mao J Z, Yuan T Q. Journal of Agricultural and Food Chemistry, 2013, 61: 5361. [5] Agarwal U P, McSweeny J D, Ralph S A. Journal of Wood Chemistry and Technology, 2011, 31(4): 324. [6] Bjrkman A. Nature, 1954, 174: 1057. [7] Lupoi J S, Smith E A. Applied Spectroscopy, 2012, 66(8): 903. [8] Schrader B, Klump H H, Schenzel K, et al. Journal of Molecular Structure, 1999, 509(1): 201. [9] Sun L, Varanasi P, Yang F. Biotechnology and Bioengineering, 2012, 109(3): 647. [10] Agarwal U P, Reiner R S, Pandey A K, et al. Raman Spectra of Lignin Model Compounds//Proc. 13th Inter. Symp. Wood, Fiber, and Pulping Chemistry. Vol. 1, Carlton, Australia: Appita, 2005. 1. [11] Stalikas C D. Journal of Separation Science, 2007, 30(18): 3268. [12] HU Zheng-rong, LIU Yu-huan, RUAN Rong-sheng(胡铮瑢, 刘玉环, 阮榕生). Food Science(食品科学), 2009, 30(21): 438. [13] Kim H, Ralph J. Organic and Biomolecular Chemistry, 2010, 8: 576. [14] Wen J L, Xue B L, Xu F. BioEnergy Research, 2012, 5(4): 886.
