Abstract:In order to realize the rapid determination of ractopamine content in pork, quantitative determination model of ractopamine content in pork was established by using three-dimensional synchronous fluorescence spectrum coupled with alternating penalty trilinear decomposition (APTLD). Firstly, the generation mechanism of the fluorescence spectrum for ractopamine and three-dimensional synchronous fluorescence spectrum for samples were analyzed. Secondly, concentration quenching phenomenon of fluorescence of ractopamine in pork extract was investigated. Thirdly, the number of components for three linear decomposition of APTLD was set as 2 by using the core consistency diagnostic method, and the calibration curve of the relative fluorescence intensity of ractopamine between pork extract and the training sample was established for the correction of relative fluorescence intensity of prediction samples. Finally, three-dimensional synchronous fluorescence spectrum combined with APTLD was used to build the prediction model of ractopamine content in pork. The experimental results showed that the method adopted in the paper could better solve the problem of serious synchronous fluorescence spectrum overlapping between ractopamine in pork samples and backgrounds, and leave out some trivial process of chemical separation for the identification of ractopamine in pork. The determination coefficient (R2) and the root mean squared error of prediction (RMSEP) for the model proposed in this paper were 0.986 3 and 0.496 6 mg·L-1, respectively. The method in this paper has achieved the goal of rapid quantitative detection of ractopamine content in pork.
[1] WANG Di, YANG Shu-ming, LIU Xiao-wei, et al(王 迪, 杨曙明, 刘潇威, 等). Journal of Instrumental Analysis(分析测试学报), 2010,29(8): 812. [2] HU Lei,ZUO Peng,YE Bang-ce(胡 磊,左 鹏,叶邦策). Contemporary Chemical Industry(当代化工), 2010,39(4): 481. [3] CHENG Sheng-hua, YANG Chun-liang, ZHENG Long, et al(程盛华,杨春亮,郑 龙, 等). Analytical Instrumentation(分析仪器), 2009,(4): 69. [4] Chu Qingcui, Geng Chenghuai, Zhou Hui, et al. Chinese Journal of Chemistry, 2007,25(12): 1832. [5] He Limin, Su Yijuan, Zeng Zhenling, et al. Animal Feed Science and Technology, 2007, 132(3-4): 316. [6] Wang J P, Zhang S X, Shen J Z. Journal of Animal Science, 2006, 84 (5): 1248. [7] Shen Jianzhong, Zhang Zhen, Yao Yan, et al. Anal. Bioanal. Chem., 2007, 387(4): 1561. [8] WANG Xue-mei, WU Hai-long, NIE Jin-fang, et al(王雪梅, 吴海龙, 聂瑾芳, 等). Chinese Journal of Analytical Chemistry(分析化学), 2009, 37(6):811. [9] YU Li-li,WU Hai-long,FU Hai-yan, et al(于丽丽, 吴海龙, 付海燕, 等). Chinese Journal of Analytical Chemistry(分析化学), 2011, 39(1):27. [10] YIN Chun-ling, HU Le-qian, YANG Xi-ping, et al(尹春玲, 胡乐乾, 杨喜平,等). Journal of Henan University of Technology·Natural Science Edition(河南工业大学学报·自然科学版),2007,28(4):41. [11] Lü Gui-cai, ZHAO Wei-hong, WANG Jiang-tao(吕桂才,赵卫红,王江涛). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(8):1144. [12] FAN Xiao-bo,YANG Jin-yi, GAO Xiu-jie, et al(樊晓博,杨金易,高秀杰, 等). Food Science(食品科学), 2010, 31(20):307. [13] LI Hong-bin, LIU Wen-qing, WANG Zhi-gang, et al(李宏斌,刘文清,王志刚,等). Chinese Journal of of Quantum Electronics(量子电子学报), 2007, 24(3):306.