Discrimination of Bamboo Using FTIR Spectroscopy and Statistical Analysis
LI Lun1, LIU Gang2*, ZHANG Chuan-yun2, OU Quan-hong2, ZHANG Li2, ZHAO Xing-xiang2
1. Key Laboratory of Molecular Spectroscopy, Colleges and Universities in Yunnan Province, Chuxiong Normal University，Chuxiong 675000, China 2. School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500, China
Abstract：Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used to identify and classify bamboo leaves. FTIR spectra of fifty-four bamboo leaf samples belonging to six species were obtained. The results showed that the infrared spectra of bamboo leaves were similar, and mainly composed of the bands of polysaccharides, protein and lipids. The original spectra exhibit minor differences in the region of 1 800~700cm-1. The second derivative spectra show apparent differences in the same region. Principal component analysis and hierarchical cluster analysis were performed on the second derivative infrared spectra in the range from 1 800 to 700 cm-1. The leaf samples were separated into 6 groups with accuracy of 98% with the first three principal components, and with 100% accuracy according to the third and fourth principal components. Hierarchical cluster analysis can correctly cluster the bamboo leaf samples. It is proved that Fourier transform infrared spectroscopy combined with PCA and HCA could be used to discriminate bamboo at species level with only a tiny leaf sample.
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