Studies on Thermal Denaturation of Peanut Allergen Ara h1 and its Interaction with Reducing Sugars
XU Hong1, SHEN Liang-liang1, HU Zhang-li1*, XIAO Jie3*, XIAO Hua-xin2, WU Jin-xia2, LI Yi1, WEI Bo3, NI Zhuo3, WU Xu-li4, LIU Zhi-gang4
1. College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China 2. College of Life Sciences, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060, China 3. College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, China 4. College of Medical Science, Shenzhen University, Shenzhen 518060, China
Abstract：The thermal denaturation of peanut allergen Ara h1, its interaction with reducing sugars and the corresponding changes in allergenicity were investigated by circular dichroism(CD), fluorescence and ELISA method comprehensively. The experimental results indicate that the secondary structure of Ara h1 changes significantly along with decreasing α-helical structure and its allergenicity with the temperature higher than 85 ℃, and that both xylose and fructose can stabilize Ara h1 protein structure through interacting with Ara h1 protein and decrease its allergenicity obviously. This study should be helpful to the further understanding of sensitization mechanism of food allergy and be useful for the guidance on reasonable manufacturing of peanut foods.
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