Study on Mechanism of Lanthanide Sensitized Fluorescence Energy Transfer between Terbium(Ⅲ)-Ciprofloxacin and Rhodamine B
LIU Bao-sheng1, WANG Yun-ke1,ZHANG Yan-qing1,FU Li2,Lü Yun-kai1
1. Center of Physics and Chemistry, Hebei University, Baoding 071002, China 2. Department of Chemistry，Langfang Teachers College, Langfang 065000, China
Abstract：A complex composed of ciprofloxacin and terbium (Tb3+) in the solution of acetic acid-sodium acetate buffer (pH 6.2) was studied by fluorescence spectroscopy and ultra-violet absorption spectroscopy. The emission peak of Tb3+ appeared at 490, 545, 590 nm, (the sensitized fluorescence was enforced mightily)and the intensity of 545 nm emission peak was increased obviously. In its acute emission spectrum, the strongest emission peak of Tb3+ was at 545 nm, which was close to the wavelength of the biggest absorption peak of RB, 552 nm. Therefore, as the basic dye rhodamine B(RB) was added, the fluorescence intensity of 545 nm emission peak decreased regularly, indicating that there was a great quenching process. The result showed that the course was statistic. Based on the mechanism of the Frster energy transfer, the efficiency of energy transfer and the distance between the acceptor RB and the complex were found. Thereby, it was indicated that the course of action was single static quenching and the mechanism of quenching was based on energy transfer.
Key words：Ciprofloxacin;Terbium;Rhodamine B;Fluorescence resonance;Energy transfer
刘保生1,王云科1,张彦青1,傅丽2,吕运开1 . 铽-环丙沙星与罗丹明B间稀土敏化荧光能量转移机理研究[J]. 光谱学与光谱分析, 2008, 28(01): 153-155.
LIU Bao-sheng1, WANG Yun-ke1,ZHANG Yan-qing1,FU Li2,Lü Yun-kai1 . Study on Mechanism of Lanthanide Sensitized Fluorescence Energy Transfer between Terbium(Ⅲ)-Ciprofloxacin and Rhodamine B. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(01): 153-155.
[1] Arnaud N, Georges J. Analyst, 1997, 122(2): 143. [2] Diamandis P. Analyst, 1992, 117(12): 1879. [3] WANG Lei, HAN Ben-zheng, SANG Li-hong, et al(王 磊, 韩本政, 桑立红, 等). Chinese Journal of Pharmaceuticals(中国医药工业杂志),2000, 31(11): 508. [4] LU Yan, ZHAO Hui-chun, SUN Cun-yan, et al(陆 燕, 赵慧春, 孙春燕, 等). Acta Pharmaceutica Sinica(药学学报),2002, 37(11): 876. [5] WANG Jing-ping, DU Li-ming, DUAN Ya-li(王静萍, 杜黎明, 段亚丽). Chinese Journal of Analytical Chemistry(分析化学),2004, 32(1): 50. [6] QI Xue-yong, HE Hua, GU Fei, et al(戚雪勇, 何 华, 顾 飞, 等). Chinese Pharmaceutical Journal(中国药学杂志),2005, 40(10): 1500. [7] TONG Shang-lun, XIANG Guang-hong, LIU Wei-ping(童裳伦, 项光宏, 刘维屏). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(12): 2061. [8] WU Shu-qing, CAO Qiu-e, ZHAO Yun-kun, et al(吴淑清, 曹秋娥, 赵云昆, 等). Chinese J. Anal. Chem.(分析化学),2000,28(12): 1462. [9] LIU Bao-sheng, ZHANG Hong-yi, WANG Fu-li(刘保生, 张红医, 王甫丽). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(3): 539.
