Determination of Sparfloxacin Concentrations in Chicken Serums and Residues in Chicken Tissues and Manures Using Self-Ordered Ring Fluorescence Microscopic Imaging Technique
DONG Cheng-yu, LIU Yuan-yuan, LIU Ying*
College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
Determination of Sparfloxacin Concentrations in Chicken Serums and Residues in Chicken Tissues and Manures Using Self-Ordered Ring Fluorescence Microscopic Imaging Technique
DONG Cheng-yu, LIU Yuan-yuan, LIU Ying*
College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
摘要: Based on the self-ordered ring (SOR) fluorescence microscopic imaging technique on a hydrophobic glass slide with Zn2+ and cetyltrimethyl ammonium bromide (CTMAB) as sensitizer, and poly (vinyl alcohol) -124 (PVA-124) and NH3-NH4Cl (pH 10.00) as the medium, a method has been developed for determining sparfloxacin (SPFX) concentrations in chicken serum and residues in chicken tissues and manures. When the droplet volume was 0.20 μL, SPFX was determined in the range of 1.38×10-13~2.03×10-12 mol·ring-1 (or 6.90×10-7~1.02×10-5 mol·L-1), and the limit of detection (LOD) was 14 fmol·ring-1 (or 6.90×10-8 mol·L-1). The recoveries of SPFX at all different spiked levels are in the range of 90.74%~106.61% when the methanol or acetonitrile were used as extracting agent, respectively, and the relative standard deviations (RSDs) are less than 3.0%. This study expands the applied fields of SOR technique in drug concentrations and residues determination.
Abstract:Based on the self-ordered ring (SOR) fluorescence microscopic imaging technique on a hydrophobic glass slide with Zn2+ and cetyltrimethyl ammonium bromide (CTMAB) as sensitizer, and poly (vinyl alcohol) -124 (PVA-124) and NH3-NH4Cl (pH 10.00) as the medium, a method has been developed for determining sparfloxacin (SPFX) concentrations in chicken serum and residues in chicken tissues and manures. When the droplet volume was 0.20 μL, SPFX was determined in the range of 1.38×10-13~2.03×10-12 mol·ring-1 (or 6.90×10-7~1.02×10-5 mol·L-1), and the limit of detection (LOD) was 14 fmol·ring-1 (or 6.90×10-8 mol·L-1). The recoveries of SPFX at all different spiked levels are in the range of 90.74%~106.61% when the methanol or acetonitrile were used as extracting agent, respectively, and the relative standard deviations (RSDs) are less than 3.0%. This study expands the applied fields of SOR technique in drug concentrations and residues determination.
DONG Cheng-yu, LIU Yuan-yuan, LIU Ying* . Determination of Sparfloxacin Concentrations in Chicken Serums and Residues in Chicken Tissues and Manures Using Self-Ordered Ring Fluorescence Microscopic Imaging Technique [J]. 光谱学与光谱分析, 2012, 32(10): 2759-2764.
DONG Cheng-yu, LIU Yuan-yuan, LIU Ying* . Determination of Sparfloxacin Concentrations in Chicken Serums and Residues in Chicken Tissues and Manures Using Self-Ordered Ring Fluorescence Microscopic Imaging Technique . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(10): 2759-2764.
[1] Phillips I, Casewell M, Cox T, et al. J. Antimicrob. Chemother., 2004, 53(1): 28. [2] Horii T, Monji A, Uemura K, et al. J. Microbiol. Methods., 2006, 65(3): 557. [3] Zhao L, Dong Y H, Wang H. Sci. Total Environ., 2010, 408(5): 1069. [4] Tagawa M, Kano M, Okamura N, et al. Br. J. Clin. Pharmacol., 2002, 53(3): 296. [5] You Q D. Medicinal Chemistry. Beijing: Chemical Industry Press, 2008. 341. [6] Mody V D, Pandya K K, Satia M C, et al. J. Pharm. Biomed. Anal., 1998, 16(8): 1289. [7] Hernández-Arteseros J A, Barbosa J, Compaó R, et al. J. Chromatogr. A, 2002, 945(1-2): 1. [8] Zhao S, Jiang H Y, Li X L, et al. J. Agric. Food. Chem., 2007, 55(10): 3829. [9] Zhao S J, Li X L, Ra Y, et al. J. Agric. Food. Chem., 2009, 57(2): 365. [10] Lolo M, Pedreira S, Fente C, et al. J. Agric. Food. Chem., 2005, 53(8): 2849. [11] Toussaint B, Chedin M, Vincent U, et al. J. Chromatogr. A, 2005, 1088(1-2): 40. [12] Heller D N, Nochetto C B, Rummel N G, et al. J. Agric. Food. Chem., 2006, 54(15): 5267. [13] McCourt J, Bordin G, Rodriguez A R. J. Chromatogr. A, 2003, 990(1-2): 259. [14] Huet A C, Charlier C, Tittlemier S A, et al. J. Agric. Food. Chem., 2006, 54(8): 2822. [15] Liu Y, Huang C Z, Li Y F. Anal. Chem., 2002, 74(21): 5564. [16] Yu Y M, Liu Y. J. Anal. Chem., 2011, 66(8): 728. [17] Li Y F, Huang C Z. Chem. Lett., 2002, 31(12): 1192. [18] Huang C Z, Liu Y, Li Y F. J. Pharm. Biomed. Anal., 2004, 34(1): 103.