Abstract：The conformation changes of Apo-Ovotransferrin and Holo-Ovotransferrin were studied with the heat treatment 25~95 ℃ by using Fourier transform infrared spectroscopy (FTIR) and two-dimensional correlation spectroscopy analyzer. The results of one-dimensional infrared spectroscopy showed that with the increase in temperature, the peak at 3 300 cm-1 of Apo-Ovotransferrin shifted more than that of Holo-Ovotransferrin. The peak at 3 300 cm-1 derived from stretching vibrations of N—H and O—H indicates that iron-binding enhanced the role of hydrogen bonds and resistance to heat. The changing order of the secondary structure of ovotransferrin was determined by analyzing two-dimensional infrared spectra，witch is β-sheet>amideⅡ>—CH2— bending vibration. In addition, it was found that the cross-peaks at 1 652 and 1 688 cm-1 are different in synchronous and asynchronous counter maps by comparing Apo-Ovotransferrin with Holo-Ovotransferrin. It was suggested that the temperature made less impact on the α-helix in Holo-Ovotransferrin than on that in Apo-Ovotransferrin, however, the β-turn in Holo-Ovotransferrin was more sensitive to temperature.
Key words：Two-dimensional infrared correlation spectroscopy;Temperature;Ovotransferrin;Hydrogen bond
任国栋，郭爱玲，耿 放，马美湖*，黄 群，武小芬 . 二维红外相关光谱研究温度对卵转铁蛋白构象的影响[J]. 光谱学与光谱分析, 2012, 32(07): 1780-1784.
REN Guo-dong, GUO Ai-ling, GENG Fang, MA Mei-hu*, HUANG Qun, WU Xiao-fen . Study of the Effect of Temperature on the Conformation of Ovotransferrin by Two-Dimensional Infrared Correlation Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(07): 1780-1784.
[1] Mizutani K, Muralidhara B K, Yamashita H, et al. J. Biol. Chem., 2001, 276(38): 35940. [2] YUAN Xiao-jun，MA Mei-hu，HUANG Qun, et al(袁小军，马美湖，黄 群, 等). Food & Machinery(食品与机械), 2011, 27(2): 125. [3] TONG Ping，GAO Jin-yan，CHEN Hong-bing(佟 平，高金燕，陈红兵). Science and Technology of Food Industry(食品工业科技), 2010, 31: 100. [4] Mizutani K, Yamashita H, Kurokawa H, et al. J. Biol Chem., 1999, 274(15): 10190. [5] Dewan J C, Mikami B, Hirose M, et al. Biochemistry, 1993, 32(45): 11963. [6] Kurokawa H, Mikami B, Hirose M. J. Mol. Biol., 1995, 254(2): 196. [7] Kurokawa H, Dewan J C, Mikami B, et al. J. Biol. Chem., 1999, 274(40): 28445. [8] Mizutani K, Mikami B, Hirose M. J. Mol. Biol., 2001, 309(4): 937. [9] Ibrahim H R, Iwamori E, Sugimoto Y, et al. Biochim. Biophys. Acta, 1998, 1401(3): 289. [10] Ko K Y, Mendonca A F, Ahn D U. Poultry Science, 2008, 87(12): 2660. [11] Biver T, Friani R, Gattai C, et al. J. Phys. Chem. B, 2008, 112(38): 12168. [12] Taniguchi T, Ichimura K, Kawashima S, et al. Eur. Biophys J., 1990, 18(1): 1. [13] Noda I. Journal of the American Chemical Society, 1989, 111(21): 8116. [14] Noda I. Society for Applied Spectroscopy, 1993, 47(9): 1329. [15] Croguennec T, Nau F, Pezennec S, et al. European Food Research and Technology, 2001, 212(3): 296. [16] Noda I. Society for Applied Spectroscopy, 1990, 44(4): 550. [17] Barth A. Biochim Biophys Acta, 2007, 1767(9): 1073. [18] TAN Qing-tian，TIAN Zhen-hua，LI Guo-ying(谭擎天，田振华，李国英). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2011, 31(4): 970.
