Investigation of the Enrichment and Separation of Lead and Lead Isotopes in Simulated Blood Matrix
WU Jing1，4, OUYANG Li1，3*, TANG Xiao-ping2, LIU Tie-bing1, LIU Duo-jian1, WANG Jing-yu1，3
1. School of Public Health, Peking University, Beijing, 100191, China 2. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China 3. Laboratory of Biometallomics Center of Medical & Health Analysis, Peking University, Beijing 100191, China 4. School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China
Abstract：The enrichment and separation method was used in the present paper to overcome the influence of matrix effect on the determination of lead concentration and isotope ratios. The patented method was used to enrich and separate the lead in simulated blood matrix; the lead concentrations and isotope ratios were determined by ICP-MS before and after enrichment and separation. The recovery rate of Pb is more than 99% through this method while the removal rate of major impurity ions such as Na, K is about 80%. The concentrations of each lead isotope decrease with the matrix concentration increasing (p<0.001), and lead isotope ratios show no significant differences in the matrix of different concentrations. When the matrix concentration is more than 62.5 μg·mL-1, the strength will decrease with the determination times increasing. It was concluded that the characteristics of this method are of high recovery rate (>99%), fast, simple and convenient, and it can raise the accuracy and precision of the concentration determination of lead isotopes and has no significant influence on the accuracy of lead isotope ratios determination.
Key words：Enrichment and separation;Matrix effect;Lead;Isotope ratio
吴 晶1, 4，欧阳荔1, 3*，唐晓萍2，刘铁兵1，刘多见1，王京宇1, 3 . 模拟全血基体中铅及其同位素的富集与分离研究[J]. 光谱学与光谱分析, 2012, 32(03): 765-769.
WU Jing1，4, OUYANG Li1，3*, TANG Xiao-ping2, LIU Tie-bing1, LIU Duo-jian1, WANG Jing-yu1，3 . Investigation of the Enrichment and Separation of Lead and Lead Isotopes in Simulated Blood Matrix . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(03): 765-769.
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