Analysis of Pigments from Rhodotorula Glutinis by Raman Spectroscopy and Thin Layer Chromatography
YUAN Yu-feng1, 2, TAO Zhan-hua2, WANG Xue1, 2, LI Yong-qing3, LIU Jun-xian1*
1. College of Physics and Technology, Guangxi Normal University, Guilin 541004, China 2. Lab of Biophysics of Guangxi Academy of Sciences, Nanning 530003, China 3. Department of Physics, East Carolina University, Greenville, North Carolina 27858-4353, USA
Abstract：The pigments from Rhodotorula glutinis were separated by using thin layer chromatography, and the result showed that Rhodotorula glutinis cells could synthesize at least three kinds of pigments, which were beta-carotene, torulene, and torularhodin. The Raman spectra based on the three pigments were acquired, and original spectra were preprocessed by background elimination, baseline correction, and three-point-smoothing, then the averaged spectra from different pigments were investigated, and the result indicated that Raman shift which represents CC bond was different, and the wave number of beta-carotene demonstrated the largest deviation, finally torulene and torularhodin in Rhodotorula glutinis had more content than beta-carotene. Quantitative analysis of Raman peak height ratio revealed that peak height ratio of pigments showed little difference, which could be used as parameters for further research on living cells, providing reference content of pigments. The above results suggest that Raman spectroscopy combined with thin layer chromatography can be applied to analyze pigments from Rhodotorula glutinis, provides abundant information about pigments, and serves as an effective method to study pigments.
Key words：Raman spectroscopy;Thin layer chromatography;Rhodotorula glutinis pigments;Raman peak height ratio
袁玉峰1, 2, 陶站华2，王 雪1, 2，黎永青3，刘军贤1* . 拉曼光谱法结合薄层层析分析红酵母色素[J]. 光谱学与光谱分析, 2012, 32(03): 695-698.
YUAN Yu-feng1, 2, TAO Zhan-hua2, WANG Xue1, 2, LI Yong-qing3, LIU Jun-xian1* . Analysis of Pigments from Rhodotorula Glutinis by Raman Spectroscopy and Thin Layer Chromatography . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(03): 695-698.
[1] Simpson K L, Nakayama T O, Chichester C O. J. Bacteriol., 1964, 88(6): 1690. [2] Sakaki H, Nochide H, Komemushi S, et al. J. Biosci. Bioeng., 2002, 93(3): 339. [3] Spano F C. Am. Chem. Soc., 2009, 131(12): 4267. [4] Serino S, Gomez L, Costagliola G, et al. Agric. Food Chem., 2009, 57(19): 8753. [5] Tao Z H, Wang G W, Xu X D, et al. FEMS Microbiology Letters, 2011, 314(1): 42. [6] Puppels G J, Olminkhof J H, Segers-Nolten G M, et al. Exp. Cell Res., 1991, 195(2): 361. [7] Dresselhaus M S, Jorio A, Filho A G, et al. Phil. Trans. R. Soc. A, 2010, 368(1932): 5355. [8] Wang G W, Zhang P F, Peter Setlow, et al. Applied and Environmental Microbiology, 2011, 77(10): 3368. [9] Wu H W, Volponi J V, Oliver A E, et al. PNAS, 2011, 108(9): 3809. [10] Xie C, Mace J, Dinno M A, et al. Anal. Chem., 2005, 77(14): 4390. [11] Bhosale P B, Gadre R V. Appl. Microbiol. Biotechnol., 2001, 55(4): 423. [12] Perrier V, Dubreucq E, Galzy P. Arch. Microbiol., 1995, 164(3): 175. [13] Schulz H, Baranska M, Baranski R. Biopolymers, 2005, 77(4): 213. [14] Klyne W, Vbeibel S, Bodea C, et al. Journal of Biological Chemisry, 1972, 247(9): 2639.
