九州虫草不同部位的红外光谱分析与评价

doi:10.3964/j.issn.1000-0593(2011)09-2420-04

摘要
参考文献
相关文章 (15)

全文: PDF (1533 KB)
输出: BibTeX | EndNote (RIS)

摘要：利用傅里叶变换红外光谱、二阶导数谱首次对九州虫草不同部位进行了分析及评价研究。结果表明，九州虫草含有蛋白、多糖、核苷、脂类等活性成分，各部位的一维谱图相似度较高，人工子座与培养基之间的相似度高达0.992 7，野生子座与虫体的差异则较为明显，二者相似系数为0.949 9。二阶导数谱进一步丰富和确认了相应谱峰特征，证明了活性物质虫草素及腺苷的存在，提示了虫草多糖α-型和β-型糖苷键的存在。红外光谱图综合反映了虫草不同部位化学成分的多样性和复杂性，为虫草各部位的细微差异分析和评价及后期质控提供了快速、全面和客观的方法和手段，为九州虫草种质资源的开发利用提供了理论基础。

关键词：九州虫草;傅里叶变换红外光谱;二阶导数谱;质量评价

Abstract：Fourier-transform infrared spectroscopy (FTIR) and second derivative spectra were used to analyze and evaluate the different parts of Cordyceps kyushuensis Kob in the present work. The results showed that C. kyushuensis contained proteins, polysaccharides, nucleosides, lipids and other active ingredients, the single-dimensional IR spectra of the various parts were highly similar, the similarity coefficient between the cultured stroma and medium reached up to 0.992 7, and the natural stroma was more different from the parasites, with similarity coefficient of 0.949 9. Second derivative spectrum further enriched and confirmed the feature of corresponding spectrum peaks, proved the existence of active substances such as cordycepin and adenosine, and prompted the presence of α- and β-glycosidic bonds. The diversity and complexity of chemical constituents in different parts of C. kyushuensis were synthetically described by IR spectra, which provided a fast, comprehensive and objective approach to the analysis and evaluation of the imperceptible differences, and the quality control of Cordyceps. This work supplies a theoretical basis for development and utilization of genetic resources C. kyushuensis.

Key words：Cordyceps kyushuensis;Fourier-transform infrared spectroscopy;Second derivative spectra;Quality assessment

收稿日期: 2010-10-23 修订日期: 2011-02-20

中图分类号:

O657.3

通讯作者: 凌建亚 E-mail: lingjian-ya@sdu.edu.cn

引用本文:

宫本佼^{1, 2}，容蓉²，张国英^{1, 2}，凌建亚^1*，张长铠¹ . 九州虫草不同部位的红外光谱分析与评价[J]. 光谱学与光谱分析, 2011, 31(09): 2420-2423.
GONG Ben-jiao^{1, 2}, RONG Rong², ZHANG Guo-ying^{1, 2}, LING Jian-ya^1*, ZHANG Chang-kai¹ . Analysis and Evaluation of Different Radial Parts of Cordyceps Kyushuensis by Fourier Transform Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(09): 2420-2423.

链接本文:

https://www.gpxygpfx.com/CN/10.3964/j.issn.1000-0593(2011)09-2420-04 或 https://www.gpxygpfx.com/CN/Y2011/V31/I09/2420

[1] LING Jian-ya(凌建亚). Identification of Anamorph of Cordyceps Kyushuensis, Phylogenetic Relationships in the Genus Cordyceps and Interaction of Cordycepin on Calf-thymus DNA(九州虫草无性型鉴定暨虫草属分子系统学及虫草素与DNA直接作用机理研究). Jinan: Shandong University(济南: 山东大学), 2003.
[2] Ling Jianya, Sun Yingjie, Zhang Hua, et al. Journal of Bioscience and Bioengineering, 2002, 94(4): 371.
[3] Ling Jianya, Zhang Guoying, Lin Jianqun, et al. Separation and Purification Technology, 2009, (66): 625.
[4] SUN Ying-jie(孙迎节). Study on the Medicinal Potential of Cordyceps Kyushuensis Found from Mengshan and the Molecular Mechanisms of Carcinoma Cell Apoptosis Induced by It(九州虫草药用价值及其诱导肿瘤细胞凋亡的分子机理研究). Jinan: Shandong University(济南: 山东大学), 2003.
[5] ZHANG Gui-jun(张贵君). Identification of Traditional Chinese Medicine(TCM)(中药鉴定学). Beijing: Science Press(北京: 科学出版社), 2002. 297.
[6] ZHOU Yu-xin(周玉新). The Modern Identify Technique of Chinese Herbal Medicines(现代中药鉴定技术). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2004. 230.
[7] SUN Su-qin, DU De-guo, LIANG Xi-yun(孙素琴, 杜得国, 梁曦云). Chinese Journal of Analytical Chemistry(分析化学), 2001, 29(3): 309.
[8] SUN Su-qin, LIANG Xi-yun, YANG Xian-rong(孙素琴, 梁曦云, 杨显荣). Chinese Journal of Analytical Chemistry(分析化学), 2001, 29(5): 552.
[9] Yang Ping, Song Ping, Sun Su-qin, et al. Spectrochimica Acta Part A, 2009, (74): 983.
[10] ZHANG Guang-qiang, HUANG Shi-de(张广强, 黄世德). Analysis Chemistry(分析化学). Beijing: Xue Yuan Press(北京: 学苑出版社), 2004. 100.
[11] WENG Shi-fu(翁诗甫). Fourier Transform Infrared Spectrometer(傅里叶变换红外光谱仪). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. 320.
[12] NING Yong-cheng(宁永成). Structural Identification of Organic Compounds and Organic Spectroscopy(有机化合物结构鉴定与有机波谱学). Beijing: Science Press(北京: 科学出版社), 2001. 485.
[13] Sandula J, Kogan G, Kacurakova M, et al. Carbohydrate Polymers, 1999, 38(3): 247.
[14] Kacurakov M, Capek P, Sasinkova V, et al. Carbohydrate Polymers, 2000, 43(2): 195.
[15] Gutierrez A, Prieto A, Martinez A T. Carbohydrate Research, 1996, 281(1): 143.
[16] Kirk P M, Cannoll P F, David J C. Ainsworth ＆ Bisby’ Dictionary the Fungi. 9th edn. Wallingfald: CAB Publishing, 2001.
[17] CABI Bioscience and CBS Database Fungal of Names [DB/OL]. http://www. index fungorum.org.