Spectroscopy Study on the Selectively Distinguishing Cefalexin with the Molecular Imprinted Polymer
JIANG Yu-xiang1, PANG Dao-lin1, HE Qiang2, LAI Cheng-yue1, ZHENG Huai-li2, LI Fang1*
1. College of Chemistry, Key Laboratory of Green Chemistry and Technology, Ministry of Education, Sichuan University, Chengdu 610064, China 2. Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China
Abstract:Molecular imprinted polymers of cefalexin (CFL) were prepared by non-covalent molecular imprinting technique in the present paper. Using CFL as template molecule, acrylamide (AM) or methacrylic acid (MAA) as functional monomer, ethylene dimethacrylate (EGDMA) as cross-linker, AIBN as initiator and methanol as porogen agent,different molecularly imprinted polymers (MIPs) of CFL were synthesized by bulk or suspension polymerization as synthetic method. The intermolecular action between AM and CFL was investigated by UV and IR spectrophotometric analysis, and the results indicated that polymerizing functional monomer AM could bond effectively with template molecule CFL. By using UV spectrophotometric analysis method, it was found that the MIP prepared with AM-EGDMA by bulk polymerization showed the highest binding capacity for CFL. Test of selective adsorption made clear that the MIP represented more excellent identification property to CFL than to cefadroxol and ampicillin. The MIPs were used as solid-phase extraction sorbent for extraction and enrichment of CFL in actual samples. And the recoveries for CFL extraction were found to be 99.3%~99.7%(n=3), demonstrating the feasibility of the prepared MIPs for CFL extraction.
蒋宇翔1,庞道林1,何 强2,赖承钺1,郑怀礼2,李 方1* . 分子印迹技术特异性识别头孢类分子的光谱研究[J]. 光谱学与光谱分析, 2011, 31(07): 1852-1856.
JIANG Yu-xiang1, PANG Dao-lin1, HE Qiang2, LAI Cheng-yue1, ZHENG Huai-li2, LI Fang1* . Spectroscopy Study on the Selectively Distinguishing Cefalexin with the Molecular Imprinted Polymer . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(07): 1852-1856.
[1] Robert J Sarah C. Journal of Chromatography B, 2004, 804: 141. [2] Andersson H S, Karlsson J G, Piletsky S A, et al. J Chromatogr. A, 1999, 848: 39. [3] JIANG Zhong-yi, WU Hong(姜忠义,吴 洪). Molecular Imprinting Technique(分子印迹技术). Beijing:Chemical Industry Press(北京:化学工业出版社), 2003. [4] Santora B P, Larsen A O, Gagne M R. Oraganometallics, 1998, 17: 3138. [5] LIU Xu-fei, ZHOU Jie, CHEN Chang-bao(柳絮飞, 周 杰, 陈长宝). Chem. J. Chinese Universities(高等学校化学学报), 2009, 30(9): 1717. [6] Masqué N, Marcé R M, Borrull F, et al. Anal. Chem., 2000, 72: 4122. [7] Edward P C L, Stanley G W. Anal. Chem. Acta, 2003, 481: 165. [8] Kochkodan V, Weigel W, Ulbricht M. Analyst, 2001, 126: 803. [9] LIU Xiao-fang, LI Feng, YAO Bing, et al(刘晓芳, 李 锋,姚 冰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2010, 30(8): 2228. [10] WU Wu-ai, YIN Zhi-fen, WEI Jing-rui, et al(武五爱, 尹志芬, 尉景瑞, 等). Chem. J. Chinese Universities(高等学校化学学报), 2008, 29(7): 1334. [11] WANG Wen-hui,LI Ping(王文辉, 李 平). Journal of Instrumental Analysis(分析测试学报), 1999,18(4):81. [12] ZHANG Yu-zhong,WU Fang-hui,XU Wen-jie(张玉忠, 吴芳辉, 徐稳杰). Chinese Journal of Analytical Chemistry(分析化学),1999, 27(4): 430. [13] FAN Shun-li,Lü Chao,WANG Jun,et al(范顺利, 吕 超, 王 军, 等). Chinese Journal of Analysis Laboratory(分析试验室), 2001,20(6): 61. [14] QUAN Hong,BI Xiao-ping,FENG Ya-xuan,et al(全 红, 毕小平, 冯亚萱, 等). Journal of Shanxi Medical University(山西医科大学学报), 2008, 39(12): 1105. [15] TAO Fu-fang, YU Zhu-qing, HAN Xiao-feng, et al(陶福芳,于竹青,韩晓峰,等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2008, 28(9): 1512.