Abstract：Fourier transform infrared spectroscopy(FTIR)technique was applied in the early detection of corynespora spot on cucumber leaves while the symptom had not appeared. The cucumber leaves were inoculated with Corynespora cassiicola. By observing the changes in the FTIR spectra of infected cucumber leaves at various times of post-infection，three sensitive bands, 1 735, 1 545 and 1 240 cm-1 were selected for the identification of cucumber corynespora leaf spot. According to the peak areas at these sensitive bands，cucumber leaf samples infected with C. cassiicola and control uninfected leaf samples could be classified correctly. Results clearly demonstrated that the FTIR technology is an available one for the early detection of corynespora spot on cucumber leaves while the symptom has not appeared and it provides a new method for the early detection of corynespora spot.
[1] LI Bao-ju(李宝聚). China Vegetables(中国蔬菜)，2006, (1)：1. [2] Erukhimovitch V，Tsrorr L，Hazanovsky M，et al. Journal of Agricultural Technology, 2005，1(1)：145. [3] CI Yun-xiang，ZANG Kai-sai，GAO Ti-yu(慈云祥，藏凯赛，高体玉). Chemical Journal of Chinese Universities(高等学校化学学报)，2002，23(6)：1047. [4] Kusano N，Hirashima K，Kuwahara M. Journal of General Plant Pathology，2007，73(1)：66. [5] Myint M S，Johnson Y J，Tablante N L，et al. Journal of Food Microbiology，2006，23(6)：599. [6] Hein I，Flekna G，Krassning M，et al. Journal of Microbiological Methods，2006，66(3)：538. [7] WANG Hui-zhe，LI Shu-ju，HUO Zhen-rong，et al(王惠哲，李淑菊，霍振荣，等). Acta Agriculturae Boreali-Sinica(华北农学报)，2006，21(1)：105. [8] WU Di，FENG Lei，ZHANG Chuan-qing，et al(吴 迪，冯 雷，张传清，等). Journal of Infrared and Millimeter Waves(红外与毫米波学报)，2007，26(4)：269. [9] Erukhimovitch V，Tsrorr L，Hazanovsky M，et al. Applied Spectroscopy，2007，61(10)：1052. [10] Naumann D，Helm D，Labischinski H. Nature，1991，351(2)：81. [11] WU Jin-guang(吴瑾光). Modern Fourier Transform Infrared Spectroscopy and Its Applications(近代傅里叶变换红外光谱技术及应用). Beijing: Scientific and Technical Documents Publishing House(北京：科学技术文献出版社)，1994. [12] Naumann A. Analyst，2009，134(6)：1215. [13] Schmitt J，Flemming H C. International Biodeterioration & Biodegradation，1998，41(1)：1. [14] Grantick S. The Journal of Biological Chemistry，1961，236(4)：1168. [15] WENG Shi-fu(翁诗甫). Fourier Transform Infrared Spectroscopy Analysis(傅里叶变换红外光谱分析). Beijing: Chemical Industry Press(北京：化学工业出版社)，2010. 264.
