Applications of Three-Dimensional Fluorescence Spectrum of Dissolved Organic Matter to Identification of Red Tide Algae
Lü Gui-cai1, 2, ZHAO Wei-hong1*, WANG Jiang-tao2
1. Key Laboratory of Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China
Abstract：The identification techniques for 10 species of red tide algae often found in the coastal areas of China were developed by combining the three-dimensional fluorescence spectra of fluorescence dissolved organic matter (FDOM) from the cultured red tide algae with principal component analysis. Based on the results of principal component analysis, the first principal component loading spectrum of three-dimensional fluorescence spectrum was chosen as the identification characteristic spectrum for red tide algae, and the phytoplankton fluorescence characteristic spectrum band was established. Then the 10 algae species were tested using Bayesian discriminant analysis with a correct identification rate of more than 92% for Pyrrophyta on the level of species, and that of more than 75% for Bacillariophyta on the level of genus in which the correct identification rates were more than 90% for the phaeodactylum and chaetoceros. The results showed that the identification techniques for 10 species of red tide algae based on the three-dimensional fluorescence spectra of FDOM from the cultured red tide algae and principal component analysis could work well.
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