Flow Injection Analysis of Trace Amounts of Doxycycline with Inhibited Chemiluminescence Detection
LI Nian-bing1, DUAN Jian-ping2, CHEN Hong-qing2, CHEN Guo-nan1
1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002，China 2. Analytical and Testing Center, Fuzhou University, Fuzhou 350002，China
Abstract：A novel flow injection chemiluminescence method has been developed for the determination of doxycycline(DC) based on the inhibition of chemiluminescence reaction of Luminol-KMnO4 by using DC in sodium hydroxide medium. Using different concentrations of KMnO4, a series of good linear regression equations of decreased chemiluminescence intensity and the concentration of DC were obtained with different subsections in the concentration range of 0.005-5.0 μg·mL-1. The detection limit (3σ) is 2.0×10-3μg·mL-1. The method has been successfully applied to the determination of DC in a pharmaceutical preparation.
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